82 
SPOLIA ZEYLANICA. 
F. to 97° F.). The results are collected in the following 
table : — 
Chemical Substance tested. 
Time of Observation. 
30' ! 
3 hours' 
7 hours 
22 hours. 
Blood without addition of any chemical 
+4- 
44 
4 ; 
Salt solution, 1 per cent. 
44 
44 
4 1 
£ 
Distilled water 
44 
44 
4 1 
fl 
Tap water 
44 
4 
4 i 
c3 
Iron perchloride, 1 per cent. 
4 
— 
g o 
Lugol's liquid 
4 
— 
— 
•5- 
Sublimate, Woo 
— 
— 
, —■ 
173 
Vesuvin Brown, 1 per cent.... 
44 
4 
4 
i o ® . 
a S ® 
Vesuvin Brown, Woo 
4 + 
4 
4 
Methyl green, 1 per cent. ... 
4 4 
— 
— 
rrt 
^ ® O 
Methyl green Woo 
44 
4 
4 
o o 
Gentian violet, 1 per cent. ... 
— 
— 
— 
43 oa 
cl} 
Gentian violet, Woo 
4- 
— 
— 
1 a 
Methylene blue l per cent. ... 
— 
— 
— 
Methylene blue, Wo 
. — 
— 
— 
|| 
Methylene blue, W^o 
— 
— 
— 
05 
Methylene blue, jWoo 
4 
4 
43 
H 
The sign 4 + means that the parasite is actively motile ; the sign 4 means that 
the parasite is sluggishly motile ; the sign — means that the parasite has lost its 
motility. 
The Table shows that some staining substances have a very 
marked action on the vitality of the parasite. Of all the stains 
tested the methylene blue acted most effectively. A 1 per cent, 
solution of methylene blue stops at once the movements of the 
trypanosomes, which appear blue-stained and with their shape 
well preserved. Such results would suggest the use of inoculations 
of methylene blue (chromotherapy) in animals and man affected 
with trypanosomosis, inasmuch as methylene blue is little 
poisonous. Manson and Low,* however, have tried methylene 
blue inoculations in a man affected with trypanosomosis without 
getting any good result. Recently Ehrlich and Shigaf have had 
good results in animals by inoculations of a solution of a new 
stain which on account of its great affinity for the trypanosome 
they have called Tryparolh. 
Hjemosporidiosis. 
The classification of the Sporozoan parasites of the blood is not 
yet quite fixed, but for present purposes, following Laveran’s 
* Brit. Med. Journal, 1904. 
t Chromotherapeutical researches on trypanosomosis. Berlin Klinische 
Wochenschr., March 28 and April 4,1904. Reviewed in Annali ai Medicina navale 
X. 1904, p. 781. 
