STUDY OF YUCCA ANGUSTIFOLIA 133 
tile principle (0.2 per cent.) in extract (2), and with traces of 
chlorophyll in extract (3). 
Fixed oil (1) was crystalline in structure. It was soluble 
in ether, chloroform, benzole, carbon di-sulphide, and amyl 
alcohol; incompletely soluble in cold or boiling alcohol, acetic 
ether, and ammonium hydrate. It was colored pale green 
by sulphuric acid of 1.634 specific gravity, and changed to 
a bright-green color by calcium di-sulphide, but formed no 
emulsion with it. Phosphoric acid colored it yellow. The 
fixed oil was saponified, and a white soap separated. This 
was decomposed, and the fatty acids recovered. Glycerin 
was separated from the soap filtrate. 
Fixed oil (2) was dissolved with difficulty in boiling 95 per 
cent, alcohol, and hardened and discolored by absolute alcohol. 
It was not saponified. Crystalline solids were separated by 
precipitating the alcoholic solution with magnesium acetate. 
They melted at 85° C. and at 6o° C., respectively. 
Fixed oil (3) was soluble in alcohol, ether, chloroform, 
benzole, carbon di-sulphide, oil of turpentine, almond oil, 
glycerin, and slowdy soluble in acetic ether. The presence of 
free fatty acids was demonstrated. The fixed oil was colored 
dark-green by syrupy antimony chloride; on adding to it sul¬ 
phuric acid of 1.475 specific gravity, and a small quantity of 
zinc, hydrogen was generated, and the solubility of the oil in 
the acid liquid was accompanied by a rosy tint given to the 
solution. 
Fixed oil (4) was crystalline in structure. It was soluble 
in warm absolute alcohol, in cold acetic ether, chloroform, 
benzole, amyl alcohol, ether, carbon di-sulphide, and glycerin. 
It was saponified, and a white soap separated. The fixed oil 
was colored dark-brown by alcoholic ammonia, and a mix¬ 
ture of ferric chloride solution and powdered rosaniline gave 
a violet-colored reaction with it. 
These fixed oils differed in their physical characters and 
chemical reactions. This difference may be due to the pre¬ 
sence of free fatty acids and glycerides in varying proportions 
in the four parts of the plant. It is of interest to note that in 
the subterranean part of the Yucca , the oil extracted from 
