134 PLANT AND ORGANIC CHEMISTRY 
the bark was solid at the ordinary temperature; from the 
wood it was of a less solid consistency; while the yellow base 
of the leaf contained an oil quite soft, and in the green leaf 
the oil was almost fluid. 
Extract (2) contained an oil of low melting-point. It melted 
at 36° C. An alcoholic solution was fractionally precipitated 
with magnesium acetate, and three members of the fatty acid 
series were isolated. The quantities obtained were small, and 
it was impossible to do more than to take the melting-point 
of two of the purified crystalline residues. They melted at 
85° C. and at 6o° C., respectively. It is a well-known fact 
that a mixture of fat acids in certain proportions has a lower 
melting-point than those of its constituents. 
Alkaloids and volatile-alkaloids were not detected in the 
petroleum spirit extracts. 
ETHER EXTRACTS 
Extract (1), Bark 0} the Root. 
The residual powder from the petroleum spirit extraction 
was. dried until thoroughly freed from petroleum spirit. It 
was then macerated with Squibb’s stronger ether in the appa¬ 
ratus already described. The ethereal extract was filtered from 
the powder. It was a clear crimson-colored liquid, tinted by 
some red coloring matter dissolved; and acid in reaction. The 
extract was slowly evaporated at the ordinary temperature; 
white needle-shaped crystals were seen as the liquid concen¬ 
trated. The ethereal residue was of a resinous character. It 
was ruby-colored, transparent, and of a softer consistency 
than ordinary resin. Microscopically, the residue was iden¬ 
tified as a resin by its color reaction with Hanstein’s aniline 
violet solution. 1 The ethereal residue was treated with petro¬ 
leum spirit to remove any traces of fat that may have been 
extracted with it. It was heated in a small tube ; at 50° C. it 
experienced a slight change, and melted at 70° C. For a de¬ 
termination of the total solids, a definite volume of the ethereal 
extract was evaporated, dried, and weighed. 
1 Botanical Micro-Chemistry, Poulsen-Trelease, Boston, 1884, p. 59. 
