148 PLANT AND ORGANIC CHEMISTRY 
Extract (2), Wood 0) the Root. 
The residual powder from the ether treatment was dried 
and macerated with Squibb’s stronger alcohol. The alcoholic 
extract was neutral in reaction; when warm it was a clear 
reddish-golden colored liquid. On cooling, a creamy-white 
solid settled at the bottom of the flask. This substance was 
soluble in water, and was identified as saponin by the usual 
tests for it. A definite volume of the alcoholic extract was 
evaporated in a current of carbonic acid, dried, and weighed. 
The residue was incinerated in a weighed platinum crucible 
for the ash determination. 
TOTAL SOLIDS. 
Alcoholic residue dried at ioo° C 
“ “ “ iio° C 
“ “ ash.. 
The alcoholic residue was treated with cold water in which 
it was soluble. A cloudy solution was formed, and on shak¬ 
ing, it became frothy, and presented the appearance of an 
emulsion. It was allowed to stand for several days to see if 
the resinous matter separated, but the emulsion was perma¬ 
nent, as no separation had taken place. The emulsified liquid 
was agitated with acetic ether, and this solvent readily sepa¬ 
rated most of the resin from the aqueous portion. The water 
extract was then evaporated to dryness and redissolved in 
water. Gelatine and alum solution did not precipitate the ex¬ 
tract, showing absence of tannin; no coloration with iron 
salts, absence of gallic acid; negative results followed tests for 
alkaloids; the aqueous extract was boiled with potash and 
no ammonia fumes were formed; adding gold chloride and 
potassio-mercuric iodide solutions to the extract gave no pre¬ 
cipitate. A measured portion of the aqueous extract was 
acidified with sulphuric acid, and agitated successively with 
petroleum spirit, benzole, and chloroform. The solvents were 
evaporated; petroleum spirit removed 0.01 per cent, of a resin¬ 
ous substance, imperfectly soluble in cold and boiling aqueous 
.14.3 per cent. 
-14.3 “ 
.00.1 “ 
