iÖ4 PLANT AND ORGANIC CHEMISTRY 
cent, alcohol, dried, and weighed, deducting ash. It con¬ 
sisted of mucilaginous substances and albuminoids. 
Weight of precipitate by 90 per cent, alcohol yielded.0.85 per cent. 
“ ash yielded.0.25 “ 
Lassaigne’s test showed the presence of albuminous sub¬ 
stances. 
The filtrate and wash alcohol from the 90 per cent, alcohol 
precipitate was evaporated to dryness, and weighed, deducting 
the amount of soda acetate. It gave 0.24 per cent. The resi¬ 
due soluble in water was mixed with acetate of copper solu¬ 
tion. A very small quantity of albuminous substances was 
precipitated by the reagent. 
The albuminoids of the bark were estimated from the total 
nitrogen in one gram of the original powder. It yielded 4.75 
per cent of albuminoids. 
The powder insoluble in dilute soda solution was washed 
with distilled water. The liquid was deeply colored. It was 
evaporated, and the amount of solids estimated. It gave 1.3 
per cent. 
Extract (2), the Wood 0} the Root. 
The powder insoluble in water was treated in the same way 
as in extract (1). The filtered solution was mixed with 90 per 
cent, alcohol in the manner described. The precipitate was 
estimated, deducting ash. 
Weight of precipitate by 90 per cent, alcohol yielded.2.170 per cent. 
“ ash yielded.0.256 “ 
The filtrate from the 90 per cent, alcohol precipitate was 
treated with water, and the soluble matter precipitated by 
copper acetate. The precipitate was collected, dried, weighed, 
and ignited, the resulting oxide of copper being deducted. It 
yielded 0.104 P er cent, of albuminoids. A current of washed 
carbonic acid was passed through the dilute soda extract to 
determine the presence of globulin (vitellin, myosin), and 
with negative results. The albuminoids were determined from 
