14 
FIG. 9—FIXING THE ORGANISMS TO THE SLIDE 
5. Lay the slide on the sink cover for staining and pour enough methylene 
blue solution on it to cover the spread as is shown in Fig. 10. 
Permit the stain to act three minutes, and then wash the preparation" with 
tap water. 
6. Dry the preparation between blotters. 
7. Label the slide, being careful to place the label at one end on the same 
side as the spread. 
Note.—This kind of a preparation answers very well for all temporary 
work where preparations are not desired for permanent use. 
In order to make the preparations more nearly permanent, the smear or 
spread may be covered with a very thin piece of glass, a cover glass, 
which may be cemented to the slide with Canada balsam. The preparation 
should be dry before the Canada balsam is placed on it. If immersion oil has 
been used on the preparation, it should be removed by flooding the spread 
