5. At the end of the periods given in Table XIV transfer a loopful of the 
suspension of each culture to a tube of sterile broth. Incubate the cultures 
48 hours at 37° C. 
6. Examine the tubes for growth, comparing them with sterile tubes if 
necessary. 
7. Construct a table on the regular note paper showing the results of the 
tests of both disinfectants, Table XV. Use a plus (+) sign when the organism 
is killed and a minus (-) sign when it is not killed. 
TABLE XV.—Test of Disinfectants 
Disinfectant 
Escherichia coli 
Bacillus Anthracis 
Exposure time in minutes 
Exposure time in hours 
3 
10 
30 
90 
1.5 
24 
48 
72 
’ 
' 
1 
What is meant by sepsis? 
What is meant by antisepsis? 
What is meant by antiseptic? 
What is a disinfectant? 
What is a germicide? 
Are all antiseptics disinfectants? 
Name two gaseous disinfectants. 
What are the advantages of gaseous disinfectants? 
Give the advantages and disadvantages of the above disin¬ 
fectants for stable disinfection. 
Give the best method of procedure in disinfecting a stable. 
What is meant by “terminal” disinfection? “concurrent” 
disinfection? 
8. (a) 
(b) 
(c) 
(d) 
(e) 
(/) 
(g) 
(h) 
(0 
0 ) 
(*) 
