APPENDIX A 
OUTLINE FOR STUDY OF BACTERIAL CHARACTERS 
A. PHYSIOLOGY (biochemical properties) 
1. Relation to oxygen 
Notice the distribution of the growth in the fermentation tubes and 
in tubes of broth and gelatin. 
2. Pigment production. 
Notice the growth on solid media. 
3. Test reaction of all sugar broths, glycerin and plain broth by remov¬ 
ing a drop with a sterile loop and placing it on litmus paper. 
4. Note gas formation in any sugar broths and in glycerin broth. If 
gas is formed in fermentation tubes, measure it and determine the 
H:C0 2 ratio as showrn by the instructor. 
5. Observe if nitrates are reduced and if gas is formed (See Exercise 17.) 
6. Test the starch agar culture for diastasic action if there is growth. 
7. Habitat. Where the organism is found. 
FIG. 22—NONLIQUEFYING GELATIN STAB CULTURES 
1 , filiform; 2, beaded; 3, echinulate; 4, villous; 5, dborescent 
