— 5i6 — 
homogeneous, non-striated, hyaline capsule, 0.024 mm. in thick- 
ness. 
Stomach : There is a parasite imbedded in the outer longitu¬ 
dinal layer of smooth muscle cells and completely surrounded by 
them just beneath the peritoneum. 
In smear préparations stained by Giemsa or Hastings’ stains, 
these sporozoites appear to be imbedded in a fine réticulum, stai- 
ning somewhat like fibrin ; but no sporoblastic chambers can be 
demonstrated. Morphologically, the parasite is definitely a repré¬ 
sentative of the genus Sarcocystis and it is one that has invaded 
a variety of tissues and organs, and has developed in voluntary 
muscle and in situations where striated muscle is not found. The 
parasites are apparentlv ail fully developed and of the same âge. 
The feeding habits of the host suggest that the parasite might, 
hâve been picked up from varions sources, very possibly birds 
and poultry. The writer has dissected fowl dying here from va¬ 
rions causes among the following varieties : 
R. I. Reds. 
Brown Leg-horns. 
Native Game. 
White Leghorns. 
Barred Plymouth Rocks and 
Black Orpingtons. 
One of the Black Orpingtons had a number of subcutaneous 
bodies, infiltrated with lime, which in size and shape were not 
unlike the sarcosporidia found in the opossum. 
Inoculation experiments. 
Animais hâve been successfully infected by sarcosporidia in ex¬ 
periments conducted by Smith (i). Nègre (2), Negri (3) and the 
writer, by feeding methods. 
On this occasion, however, an attempt was made at infection 
bv the intra-muscular inoculation of sporozoites suspended in sa¬ 
line solution. 
(1) Smith, Theobald, The Production of Sarcosporidiosis in the Mouse by 
Feeding Infected Muscular Tissue, Journal of Experimental Medicine, 1901, 
VI, I. Journal of Medical Research, 1905, XIII, 429. 
(2) Nègre, L., Sarcosporidiose Experimentale, Compt. rend. Soc. de biol., 
1907, LXIII, 374. 
(3) Negri, A., Beobachtungen uber Sarkosporidien, Cent. f. Bakt., Orig., 
1908, XLVII, 612. 
