young larvae fed on the dead bodies of flies, but never in tlie 
mature larvae, pupae or flies bred from sucli larvae. My prelimin- 
ary experim'ents bave then clearly demonstrated that Herpeto- 
monas muscae domesticae in Madras is not transmitted through 
the egg of Musca nebulo. 
As a resuit of the examination of the digestive tracts of ba- 
zaar flies (di. nebulo) it was found that they chiefly feed ou sug- 
ary foods of ail kinds and méat, and that quite a number of 
flies contain pure blood. Having ascertained these facts a select- 
ed prece of iiver on which a large number of flies were feeding 
tvas obtained from a shop. This bit of liver, which was still moisi. 
was at once placed in a stérile watch glass fixed in a tin frame, 
and then suspended in a jar containing a number of clean hat- 
ched flies which soon fed on the juice of the liver. Next day a 
number of these flies were dissected, and their alimentary tracts 
examinée! in the fresh condition, and more than 70 % were found 
to contain the flagellated forms of Herpetomonas miiscae do- 
No further observations were made on the remaining 
flies. Having made this discovery 1 decided to infect the food in 
the laboratory by allowing bazaar flies to feed on it in a clean 
jar. The procedure adopted was as follows : — fresh undamag- 
ed goat’s spleen was obtained from a shop, it was first washed 
in tap water, thoroughly dried, and then singed from three to 
four minutes in a Bunsen flame so that ail surface organisms 
were destroyed. It was then placed in a stérile Pétri dish, and 
pièces of the pulp were removed with stérile forceps and knife, 
and then placed in a stérile watch glass and suspended in a jar 
containing hundreds of wild bazaar flies, which were allowed to 
feed on the spleen pulp from one to three hours. The tin frame 
with the watch glass containing the spleen pulp was then remov¬ 
ed, ail dead flies were picked off with forceps, and the \vhole 
was suspended in another jar containing a number of clean hat- 
ched flies. The following experiment is one of many and shews 
that at least 50 % of clean bred flies may become infected with 
the flagellated forms of Herpetomonas miiscae domesticae. 
October 21 st. 1909, hundreds of wild bazaar dies were allowed !o feed on 
a piece of sterilised spleen from i-io P. M., to 2-10 P. M. At 2-15 P. M., 
the spleen was placed in a jar containing twenty two clean bred flies, which 
were allowed to feed for fifteen minutes. 
2-30 P. M. — Fly No. I. No parasites of any description found. 
Fly No. 2, Contained a number of adult flagellâtes. 
