nels to imake hlood films from the sick animais. On examininjj^ 
these préparations D'’ CîiBSOX found scveral of the animais were 
infected with piroplasmata and a few with trypanosomes. Ile im- 
mediately décidée! to inocidate ail the sick hounds evith trypan- 
blau giving’ each animal lo c. c. of a freshlv prepared 2 % solu¬ 
tion of the dye. d'he next day 1 went down to the Ken nels and 
made caretul blood films from the inoculated animais, and on 
examining then was struck by the unusual appearancés of the piro- 
plasma, as none of them shewed the typical structure of Piro- 
pJasma canis ; D'' Gibson also noted this différence. It was how- 
ever thought that the peculiar forms seen were due to the action of 
the trypanblau injected the previous evening, for Nüttall and 
IIadwex {Parasitology, Nos. i and 2, jMav 1909) hâve pointed ont 
that trypanblau exerts a marked action on PiropJasma canis de- 
stroying the typical pyriform parasites as thev escape from the 
red cells, and causing the intracellular forms to round up and 
degenerate. 
On December iith blood films were made from ail the hounds 
and immediatelv after thev were inoculated with a 2 % solution 
of trypanblau, those treated on the previous dav were given 
18 cm^ and the remainder 10 cnr^ of the dve. In the blood of 
two of the apparentlv healthv animais, Racey n°. ^and Weal- 
thy No, 33, piroplasms were found, and as their blood was taken 
before thev were inoculated, it was possible to study the mor- 
phologv of the parasites accuratelv. The parasites seen in these 
two films were in everv wav similar to those found in the animais 
alreadv inoculated with trypanblau ; the piroplasma seen on the 
morning after the first inoculation with the dye were therefore 
not altered forms. T came to the conclusion that this piroplasm 
was new to science, and conjectured that as the hounds regularly 
hunted jackals, it would probablv be found in the blood of this 
animal. 
In order therefore to prove conclusivelv that this piroplasm 
was not P. canis modified bv trypanblau, T decided to inorulate 
a number of voung bazaar dogs with defibrinated blood taken 
from one of the infected hounds. . 
Mr. H ORNE kindlv placée! ^^>althy at my disposai and on January loth 
40 cm-'* of blood was drawn from her jugular vein and at once defibrinated. 
5 cm^ of this blood was then inoculated subcutaneously into each of five 
dogs Nos. 5, 6, 7, 8 et 9, the last was at the same time given 5 cm^ o»f 
a 2 % solution of trypanblau. .Ail ticks were carefully removeJ from the 
