3o lîV 
270 
dog-s and they were kept in iron 
cages in a room in which stray ticks 
could not possibly reach them. 
Their blood and températures were 
taken twice daily. On January i5th 
a doubtful parasite was found in 
a blood film from dog No. 9 and 
on the i6th several piroplasms were 
seen, which in every way resem- 
bled those already found in the 
hounds. On the iSth one parasite 
was seen in a blood film from dog 
No. 7 ; and on the ipth one 
was found in a film from dog 
No. 6 ; on the 2oth the first para¬ 
site was found in a blood film taken 
from dog No. 8. In the case of 
dogs Nos. 5, 6, 7 and 8 no increase 
in the number of parasites was no- 
ted, but in dog No. 9 on the 2ist 
there were many parasites found in 
a blood film taken in the evening 
and bv the 26th the increase was 
still further marked. On January 
3oth an increase was also noted in 
the number of parasites in the 
blood of dogs Nos. 7 and 8, while in 
the blood of dogs Nos. 5 and 6 they 
werestill very scanty ; in dog No. 9 
thev had still further increased and 
now two, three and even four were 
found in a single red cell. On Feb- 
ruary 2nd dog No. 9 was very ill, 
its température had dropped to 95° 
F, it refused its food and died in 
the evening, its blood contained 
abondant piroplasms. On February 
41 h the blood of dog No. 7 was 
found to be swarming with paras¬ 
ites, some of the red cells containing 
from twenty to twenty five paras 
ites ; this State continued till the 6th 
w hen the dog died suddenly at 2-30 
P. M. The blood taken immédiate 
Iv after death presented a remark- 
able sight when stained in that 
there was hardly a red cell which 
did not contain a parasite, and the 
majorité harboured from two to 
thirtv. Smears of the spleen pulp 
when stained by Romanowsky’s 
stain exhibited enormous numbers 
of piroplasms so that when the film 
