w.'is exaniined with a low [)()\ver il a})peared lu bt* dolled uvcr with cuuntU'SS 
numbers of small dark dots, the nuclei of the ])arasites. The post-mortem ap- 
pearances werc shortiy as follows ; Spleen and liver mu('h enlarged, blood 
bluuk dark and very fluid, lungs, mucuus membranes and muscles extremelv 
anaemic, the latler markedly wasted. The température chart is hero reproduc- 
ed, and it will b(‘. noticed that during the course of the dis<'ase fever was not 
a marked symptom, the température never having risen above 102,4“ F. ; 
in the case of the hounds however it frequently went up to 105 to 106“ F. 
In dog No. 6 no parasites could be found for several days and as this 
dog showed some of the typical forms of Piroplasnia cavis in its blood it 
was thought that this might account for the disappearance of Piroplasnia 
gihsoui. It was therefore reinoculated subcutaneously with 6 cm® of heart 
blood from dog No. 7 and eighteen hours later several typical P. gihsoni 
were found, and on February 12 th there was a marked increase in their 
number. 
The above experinients hâve therefore proved that this new 
piroplasm found in the blood of the hounds is inoculable subcut- 
aneoLish" into bazaar dogs, and that in thent it causes the same 
disease ; a dog which lias recovered from Piroplasma canis is 
susceptible to Piroplasma gihsoni. One experiment has also 
shewn that trvpanblau has little or no effect on P. gihsoni ; this 
residt was fullv expected as repeated inoculations of the dve into 
sick hounds did not check the progre.ss of the disease. Wealthy 
333 received 40 cm^ of a 2 % solution of trvpanblau and vet 
her blood still contains many parasites. 
It now onh' remained to discover the natural source of the pa¬ 
rasite, and as mentioned above the jackal (Canis aureiis) was the 
suspected host. 
On January t 6 th I was fortunate in shooting a young jackal in broad 
day-light near the Institute well, it was immediately brought to the laborat- 
ory, so cm '■ of blood was drawn from its heart and defibrinated. Fifteen cm® 
of this was inoculated subcutaneously into each of two young bazaar dogs 
Nos. 10 and tt, and 8 cm® into a third |jup No. 12. On examining a film of 
blood from the splenic vein of the jackal a small number of red blood cells 
were found to contain the same piroplasm as was first found in the blood 
of the hounds. On January 30 th a single parasite was found in a blood film 
taken from dog No. 11 ; on several occasions in this dog’s blood a small 
number of P. canis was seen. On January 31 st. one parasite P. gihsoni 
was seen in a blood film from a pupiiy dog No. 12 and on February 4 th 
several were found in a blood film taken from dog No. 10 ; this dog had 
onlv recentlv recovered from an attack of P. canis, as its blood still contai ,i- 
ed many of the t\pical pvriform stages of this parasite. On February 
6 th blood films from dog No. lo showed manv Piroplasnia canis and a few 
Piroplasnia gihsoni, it was markedlv ill and died in the evening. The post- 
morten examination clearly showed that though Piroplasnia gihsoni was 
just begining to multiply in its blood, it died of a relapse due to Piroplasnia 
canis which was very abondant in its spleen. On February 12 th there 
was marked increase in the number of Piroplasnia gihsoni in the blood of 
dogs Nos. TI et T2. 
