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Some experiments with vaccine 
prepared according to the method 
of Achalme and Marie Phisalix 
By Philipp H. ROSS. 
For some vears glyoerinated vaccine bas been prepared at the 
laboratorv at Nairobi, British East Africa, a town situated about 
half way between Mombasa and the Lake Victoria Nyanza on 
the Uganda railway. On the whole the results with this vaccine 
hâve been as satisfactory as coulcl be expected and there has been 
little complaint from the users along the railway linè. It has been 
the custom to issue the lymph to Kisumu and Mombasa (both 
bot stations) in ice boxes, but to the intervening stations it has 
been sent without any such précaution. 
But the strip served by the railway, although at présent the 
most important area commercially of the Protectorate, is but a 
fraction of the whole from the point of view of vaccination, and 
there has been constant difficulty in getting active lymph to sta¬ 
tions far removed from the line. In one case, that of Marsabit, a 
station bordering on the Abyssinian frontier, glycerinated lymph 
was sent repeatedly but ail attempts to start vaccination with 
such lymph were a failure, the lymph being rendered inactive by 
its month’s sojourn in a hot post-bag on a porter’s head in the 
sun and at last the authorities at Marsabit had to abandon their 
efforts. 
In the Bulletin de la Société de Pathologie exotique, 190g (vol. 
II, n° 7, there appeared a paper by Achalme and Marie Phisa¬ 
lix, on the « Conservation du vaccin dans les pays chauds », and 
the results of their experiments with lymph dried and sealed in 
a partial vacuum were such that I felt sure that, if as successful 
in the field as in the laboratorv, the difficulty of supplying dis¬ 
tant stations in hot districts was solved. Accordingly I prepared 
on Nov. 2çth 1909 a portion of a collection of vaccine from the 
calf according to their methodrving over sulphuric acid in a par¬ 
tial vacuum, distributing into tubes which were also sealed in a 
partial vacuum. 
