166 — 
fed on an infected bird shortly before it died from infection with 
Fowl Pest. The tirne which elapsed between the inoculation of 
this fowl and its death from the disease was only about forty 
hours. 
These ticks were maintained at a température of about 22 0 Cent. : 
at certain intervals they were inoculated into fowls as shown 
below. 
Jan. içth. The coxal fluid of these ticks, collected immediately 
after they had finished feeding, together with the cœlomic fluid 
of one of them, were injected into a fowl. 
The resuit was négative. 
Jan. 2oth. The cœlomic fluid of one of the ticks was inoculated 
into one fowl and the contents of its alimentary canal into another. 
The first fowl remained healthy, whilst the second died from the 
disease forty hours îater. 
Jan. 22nd. The cœlomic fluid and contents of the alimentary 
canal of one of the ticks were each inoculated into a fowl. 
The bird inoculated with the contents of the alimentary canal 
died fifty-four hours Iater, whereas the other remained uninfec- 
ted. 
Jan. 2Ôth. The cœlomic fluid and gut contents of one of these 
ticks were inoculated respectively into two fowls ; whereas the bird 
inoculated with the former remained négative, the other died of 
Fowl pest sixty hours later. 
Jan. 28th. The cœlomic fluid and gut contents of one of the 
above ticks were injected respectively into two fowls. The first 
remained négative, whereas that inoculated with the contents of 
the gut died about sixty hours later. 
Feb. jrd. The cœlomic fluid and gut contents of three of the 
above ticks were inoculated respectively into two fowls. 
Botli of them remained uninfected (1). 
Feb. ioth. The remaining ten Argas were fed upon a healthy 
fowl. The latter never showed any infection with Fowl Pest. 
In the above described experiments it will be noticed that the 
virus was still alive nine days after being taken into the gut of 
the tick ; but there is a slight diminution in its virulence, as the 
fowl inoculated with the original strain died after forty hours, 
whereas after the virus had been preserved in the tick from seven 
(1) Fowl injpctpcl with eut contents, after 4 weeks interval, on March i8th, 
was inoculated with fresh virus. About 40 hours later, it died from fowl 
pest. 
