252 
A. Concentration 1 
B. Concentration 1 : 3 
C. Concentration 1 : 6 
I 
A 671 — A 651 
X 669 — X 655-5 
max. 661 
II 
A 637 — A 629 
X max. 635 
— 
III 
A 590 — A 570-5 
j a shadow from X 585 
\ X 581 — X 574 
max. 577 
IV 
A 554 — A 532 
X 551 — X 540 
„ 545 
V 
A 517 —A 486 
X 516 — X 496 
„ 504 
The colouring matter taken up by a 2 5% acid and dissolved in 
ether shows the first band much weaker than the two former sub¬ 
stances, and band V is darker than IV. The bands must therefore 
be placed according to their intensities in the following order: 
V > IV > III > I = II. 
The position of the bands shown by an. ethereal solution, whose 
concentration corresponded to solution A of the former colouring 
matter is given by the following wave lengths: 
I X 663 — 653 
II X 637 - 626 
III X 594 — 571 
IV X 556 — 532 
V X 518 — 483-5. 
The colouring matter isolated from the fraction in 5% hydro¬ 
chloric acid showed an analogous spectrum. A difference was noted 
only in the relative intensity of the two first bands. Band I appear¬ 
ed here stronger than band II. 
The phytorhodine ethers contained in the ethereal solution which 
was left after treating the original extract wdth weak alkalies, 
was treated with the view of isolating various ethers with acids of 
2 1 / 2 °/o, 5%, 7*5°/ 0 , 10% and 12% HC1. The two weakest acids 
took up but a small amount of colouring matters, whereas the 
stronger ones took up considerable quantities. The free colouring 
matters were obtained from the acid solutions by adding water 
and extracting with ether. The absorption spectra of the solutions 
obtained reminded closely of the spectra of the free phytorhodines. 
The first band in the red appeared in the stronger basic fractions 
less pronounced than in the less basic fractions, otherwise the 
spectra were quite similar. The measurement of the bands of the 
