COMMUNICATIONS 
A further experiment with vaccine prepared 
according to the method of Achalme-Phisalix 
By Philip H. ROSS. 
In a previous communication to the Society (may, 1911 ), I des- 
cribed a successful experiment with vaccine prepared according 
to the method of Achalme-Phisalix. I hâve now to lay before you 
the results of a further experiment with lymph prepared in this 
manner. 
In december 1912, I sent to D r Lowsley, medical offioer at Fort 
Hall, who had carried out the vaccinations for me in the previous 
experiment, three tubes of dried lymph. The first tube had been 
prepared on dec. 2çth. 1909, the second on jan. 6th. 1911 and the 
third on nov. 2nd. 1911. It may therefore be said tliat these three 
tubes were approximately three years, two years and one year old. 
D r Lowsley carried out the vaccinations on prisoners in the local 
gaol and his results are shown in the following table. 
Vaccina 
lions 
Not seen. 
Suc • 
cessful. 
Per cent. 
Lymph 3 years old 
23 
3 
i 4 
70 0/0 
Lymph 2 years old 
•9 
2 
i 4 
82 0/0 
Lymph 1 year old. 
26 
4 
81 0/0 
D r Lowsley notes that the patients were men (adults) and tirv- 
refore not the most favourable material for such an experiment. 
The tubes had been kept in a cardboard box on a shelf in the 
Laboratory at room température. 
I think that the results are extraordinary for lymph three years 
old and that they strongly support my view expressed in my pre¬ 
vious communication, that stations far removed from centres 
* 
where glycerinated lymph can be procured should be supplied 
