Séance du 8 Avril 1914 273 
pical sore of Cambay when injected subcutaneously or intracuta- 
neously into a susceptible animal, e. g. Macacus sinicus, lias been 
repeatedly found by the autlior (i) of this memoir to yield a néga¬ 
tive resuit, in contrast with the positive resuit invariably obtained 
by him vvhile using the virus of the disease, the beautiful lésions 
obtained by Laveran (2) in the monkey with the cultures of the pa¬ 
rasite derived from the same source, lias established the fact that 
even a culture of Leishmania tropica (Cambay) behaves exactly 
as the Tunisian virus in producing the typical lésion, at any rate 
under certain experimental conditions. It may be taken as a well 
established belief that this parasite produces only a localised lé¬ 
sion at the seat of infection and that it never généralisés itself in 
the infected animais and this belief which is generally supported 
by experimental results, lias been so strong that it is taken as an 
important pathological land mark in enabling a differential dia- 
gnosis between the two closely similar parasites viz. Leishma¬ 
nia tropica of the Oriental sore and Leishmania donovani of Kala 
Azar ; and further it lias the clinical support of the two-well mar- 
ked and distinct pictures of the disease produced by these two 
parasites in man. 
However, the interesting results recently recorded by Gon- 
der (3) go to prove that Leishmania tropica cultures when admi- 
nistered intravenously or intraperitoneally into mice, hâve always 
yielded a partially internai infection (viz. of liver and spleen but 
not bone marrow) preliminary to a localised infection periphe- 
rally, at a later period. It is in this connexion that it is found 
désirable to record the following observations on a mouse sub- 
jected to an intraperitoneal injection of Leishmania tropica cul¬ 
tures. 
The injection intraperitoneally of a Leishmania tropica culture 
into mice in one dose, being âlways found to yield a négative re¬ 
suit it was found advisable to modify the method of infection and 
administer the culture so as to produce an intensive effect on the 
mouse by injecting the material on three successive days and then 
giving a final injection after 2 months. Here are the exact details 
of the experiment. 
(1) R. Row : British Medical Journal , seot. 1910. 
<2) A. Laveran : Bulletin de la Société de Pathologie exotique, 1912. 
(3) Richard Gonder : Arch. f. Schiffs. u . Trop. Hyg , 1913. 
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