274 
Bulletin de la Société de Pathologie exotique 
Mouse (2) Leishmania tropica sériés 
Ist Injection intraperitoneally 9-4-13 1/2 c.c. 7 weeks old culture. 
2nd » » 10-4-13 0.23 cc. of 4 weeks culture. 
3rd » » 11-4-13 0.25 cc; of 4 weeks culture. 
4tli » « 11-0-13 1.23 cc. of 0 weeks culture. 
Ail cultures were ricli in 0 bodies and contained hardly any motile 
for ms. 
The animal was thriving and appeared perfectly healthy till 22.1.14 
when it was killed for observation — 9 montiis and 13 days after the tirst 
injection. 
Post mortem,tbe spleen appeared enlarged to 0 to 8 times and the liver 
distinctly so. The other organs including the peritoneum appeared per¬ 
fectly healthy. Smears made from liver, spleen and bone marrow showed 
an exceedingly rich infection. Cultures from each of tbese yielded pure 
flagellâtes and lastly the heart blood when smeared revealed a peculiar 
picture of large cocci hke dots occupying apparently the red blood cells 
singly. Could these be a « pre-parasite » phase of the Leishmania ? That 
they are not cocci is clear from their not growing on nutrient agar. 
These facts while confirming Gonder’s observation that it is 
possible to obtain a partial internai infection in mice infected in¬ 
traperitoneally with the Leishmania tropica cultures, go a step 
further and show that Leishmania tropica cultures act exactlv as 
Leishmania donovani cultures in the mouse and produce in this 
animal a generalised infection of liver, spleen, bone marrow and 
even of peripheral blood ( ?). The resuit of this experiment does 
not however confirm the observation of the grave cutaneous lésions 
he obtained in lus mice following a preliminary infection of onlv 
liver and spleen (though not of bone marrow), nor does it fend 
support to bis suggestion of the possibility of an unrecognised 
splenic or hepatic infection preliminary to the development of a 
cutaneous lésion. Ail one is impressed with, is the apparent well 
being of the mouse so heavily infected. The post mortem appea- 
rances and the microscopie examination of the organs and other 
tissues of the mouse of this experiment infected with Leishmania 
tropica cultures, being as they are identical with those of a mouse 
infected with the parasite of Kala Azar, makes one feel that the 
two parasites recognised to be so distinct from each other on ac- 
count of the two markedly different clinical pictures each proclu- 
ces in man, may be, after ail, the same parasite. In this connec¬ 
tion it may be interest ing to recal 1 the facts already recorded b y 
the author (i) that although it is usual to induce a localisée! sub- 
(i) R. Row : British Med. Journal, nov. tqi >. — Journal of Trop. Med. 
a. Hyg., nov. 1912. 
