Microscopical Society of Victoria. 
9 
(14.) The following substances have been experimented with to 
a small amount compared with the fore-mentioned, hence the 
effects cannot be spoken of so decidedly : 
The action of carbolic acid, combined with an equal bulk of 
glycerine, and a small portion of blood added on the slide, presents 
remarbable changes. The red corpuscles take various forms, some 
appear solid, some appear quite empty, or with a small portion of 
solid matter, the cell wall being in all cases thickened; some 
appear as if they had a luminous centre, this being due to the 
contraction of the contents leaving the centre empty when the 
light passes freely through it; others lose their consistence and 
become smeary, and exhibit one or more clear spaces or spots. 
This experiment can be varied by mixing magenta solution with 
carbolic acid, and then dropping on the covering glass with a small 
portion of blood on it—the magenta will colour the corpuscles in 
a pleasing way. 
(15.) The action of hydrobromic acid is peculiar; the red 
corpuscles appear as if their contents were solidified, and are seen 
in all conditions; the half empty ones with a depression on one 
side, or deeply bi-concave, and some are seen to bo solid, all 
presenting a decided pale bluish tint under feeble light. 
(16.) The action of bromal hydrate is very remarkable, differing 
from anything yet seen. All the red corpuscles are corrugated as 
to their contents, while the cell wall stands out thicker than 
usual; this corrugation of the corpuscles gives them an appearance 
of minute coscinodisci, or as if each had received the impress of a 
thimble marking. Magenta colours this very well. The addition 
of prussic acid causes a curious action; every corpuscle by degrees, 
as the acid reaches it, ceases from the corrugation of the contents, 
becomes smooth and softened in appearance. Some corpuscles are 
quite empty, presenting a decided marked cell border, very 
different from that yielded by the action of water or prussic acid 
on the globules. 
(17.) The application of nitro-glycerine solution in alcohol 
(10 per cent.) gives extraordinary results. The red corpuscles, if 
not too much acted on, appear to give up their contents and look 
emptied, and usually a nuclear like body of minute size is seen in 
them, occasionally two ; these I consider to be the solid material 
