Bums and Hedden, Conditions influencing regeneration of hypocotyl. 
385 
Adventitious buds were also produced on the epicotyl when 
the main Vegetation point was cut away. In tbis case the buds 
in the axil of the cotyledons develop, but their development 
appears to have no influence on the development op „adventi¬ 
tious“ buds. 
Material and methods. This paper deals largely witli 
conditions influencing the production of adventitious buds on 
the hypocotyl. 
The plants used were Linaria bipartita splendida. Antirrhinum 
mcijus and Linum usitatissimum. 
The cotyledons were cut away just below their insertion; 
usually when the seedling was from two to three cm high. 
After this Operation the plants were placed under various 
external conditions which will be taken up separately. 
Fig. 2. 
Linaria a b. The cotyledons were removed from a b was not injured. 
Note difference in number of leaves in regenerated sliort. Antirrhinum c and d. 
The order of shools is shown by the fignres. Note size, nnniber and arrange- 
ment of leaves in regenerated buds. 
The experiments were started in November 1903 and carried 
on through the following year. 
The results obtained confirm tliose of Küster. All indi- 
viduals of the plant narned whose cotyledons and part of the 
hypocotyl were removed produced numerous buds. 
G-eneral description. In the course of from 5 — 9 days 
after the Operation the hypocotyl buds began to appear as 
small eruption on the epidermis. A day or two before the buds 
a^ipear. the hypocotyl becomes somewhat swollen. 
The number of these buds varied in the three different 
plants Linaria produced from 1—6 buds, Antirrhinum from 1—14, 
and Linum from 1—60 or more. Every epidermal cell of Linum 
