Stop es and Fujii, The nutritive relations of the surrounding tissues etc. 7 
If we follow the history of the starch cleposition in an older 
series of ovules, for example in a series of Zamia floridana. We 
find the following conrse of events. The first deposition of starch 
takes place in the cells of the endosperm just at the base of the 
Archegonia, this spreads up the sides of the Archegonia leaving 
the jacket cells empty, but soon appearing in the neck cells where 
some grains are almost always found up tili the time of fertilization. 
In the course of the following month the amount of starch deposited 
in the endosperm cells steadily increases in quantity and in the 
size of the grains. In the jacket, tlie cells at the base of the 
archegonium are the first to be filled with starch grains and 
gradually nearly all the cells of the jacket layer become packed. 
A little later this starch is again dissolved away while yet the egg 
cell and endosperm cells are well filled with grains (cf. fig. 5), 1 ). 
fn the egg cell the starch appeared at first almost entirely at the 
perifery, and this before they are deposited in the jacket layer. 
These grains vary somewhat in size but are very much smaller 
than those in the endosperm cells, only in one exceptional case 
were they equally large. 
In egg cells ab out 2.6 to 3 mm long, the small starch grains 
frequently appeared to be associated with protein grains wliich in this 
stage were not deposited in the cells just near the egg cell but 
were present in large quantities in those somewhat removed from 
it. In further developed eggs about this size the appearance of 
the starch and protein grains was very striking. In the egg cell 
itself were present both starch and protein, the starch in very 
minute grains, the protein substance being deposited in rather 
irregulär, sometimes considerably complicated masses of very 
yarious sizes. There were very few or no protein granules in the 
jacket cells, and little in those cells of the endosperm adjoining 
the jacket layer. In the rest of the endosperm, the cells contained 
large quantities of protein, many of the grains being large and 
irregulär exactly like those of the egg cell. In the cells further 
removed from the egg cell the granules tended to become filier 
and filier, tili the region was reached in which the protein appeared 
to have filled the bulk of the cell in a viscous or semifluid 
condition which in the fixed material shewed spaces of bubble like 
appearance. Thus the deposition of protein grains began first in 
the cells near the egg, and in the egg itself as is also always the 
case with starch. ,In later stages the cells nearer the egg cell 
appeared empty of protein grains (cf. figs 7 and 8 of the same 
thing in Ginkgo) which had been re-dissolved for the use of the 
growing egg. 
A similar arrangement was seen in the case of the starch. In the 
egg cell itself there were large numbers of extremely fine roundish 
2 ) It is interesting to note that such as stage had been figured bj’ 
War min g in Ceratozamia robustä , though he did not attach any importance 
to it. Resume. ,.Rech. et. rem. s. 1. Cycadees“. Tab. II. fig. 15. (Overs, d. Kon. 
Danske Vid. Selsk. 1877.) 
