8 
Stop es and Fujii, The nutritive relations of the surrounding tissues etc. 
grains scattered throughout the whole cytoplasm as well as in a 
definite zone just at the perifery where they were so thickly 
clustered as to make an alniost solid black band ronnd the edge 
of the egg when stained with lodine. When examined with an 
oil immersion lens these small round grains appeared in general 
clustered together in groups from 2 to 10 or 20; each group 
appearing to be formed in one leucoplast (cf. fig. 6) but there 
were also a few single larger grains lying in the cytoplasm separate 
from the others. The starch grains in the egg were different in 
character and appearance from those in the endosperm cells. ln 
general they stained rather brownish violet which shews that they 
contained amylodextrine. Some of the grains in the endosperm 
cells next to the empty jacket layer were also more brownish 
staining and slnaller, probably in a partly dissolved condition, 
while the rest of the endosperm was packed with large, brightly 
bluish-violet staining grains of storage starch which later on were 
also dissolved to supply the egg. Thus the zone of emptying cells 
travels continually ontwards from the egg cells, leaving a zone of 
empty cells immediately round them. We do not find that these 
empty cells are disintegrafing or abnormal in any way, they are 
merely deprived of their own Stores and then serve as the path of 
transmission for the food stuffs from the other cells to the egg cell. 
They and their nuclei retain their integrity thronghont this stage 
of passage of food to the egg. 
Similar facts have been observed in various other species of 
Cycads. In Macrozamia spiralis however the deposition of the 
protein grains appears to preceed that of the starch, for in young 
egg cells we found many large protein grains scattered thickly 
through the whole snbstance of the egg cell, while the starch was 
only just beginning to be deposited in a few of the endosperm 
cells at the base of the Archegonia. In the jacket" cells we 
observed also small protein grains which had the appearance of so 
called “extra nucleolar nucleoli” when stained with triple stain and 
to which we will refer again. For this species of Macrozamia we 
had only alcohol material, but so far as we could jndge the sugar 
present appeared to be cane sugar, at least in the stage we 
examined, for we got many gluc-ozozone crystals formed by the 
acetate of phenylhydrazin test only when the test was made after the 
inversion process had been previously carried on. In this species 
the starch grains appear in the perifery of tbe egg rather later 
than usual. 
In later stages of all the species of Cycads examined, the 
starch again disappears from the egg cell as well as from the 
sheath cells and the immediately surrounding endosperm cells, as 
it is tnrned into soluble carbohydrates and used by the growing egg. 
In the ripening seed, the quantity of starch in the endosperm, 
as is well known, is very great except in the zone just by the egg 
cell. The cells however cannot be described as “full of starch” 
as there is such a large quantity of protein substance in definite 
grains that when tested with Millon’s reagent the endosperm 
