Stop es and Fujii, The nutritive relations of the surrounding tissues etc. 
13 
starcli in a median zone, and the pollen tubes which reached almost 
to the egg were packed witli large storage grains. In the endosperm 
cells at the base of the egg cell a few grains may have collected, 
but in the egg cell itself there were none. "Wlien the pollen tube 
had just discharged into the egg cell we found the large storage 
starcli grains from it lying together in the tip of the egg or near 
the nucleus in the upper part, and in some cases half in and half 
out of the pollen tube. A little later these big grains lay scattered 
all through the cytoplasm of the egg, having been carried round 
by a Streaming of the cytoplasm? These large grains were quite 
different in appearance from the transitory starcli usually found in 
the egg cell, and they were speedily used up by it. 
Thus there are at least three different series of starch grains 
to he found in the egg cell, a) The small grains scattered in the 
cytoplasm itself before the “nutritive vacuoles” are formed and 
which seem to be subject to daily periodicity. b) The small grains 
in the “nutritive vacuoles” the presence of which varies even among 
ovules of the same cone. c) The large grains hrought hy the 
pollen tube and speedily used up by the egg cell. All these grains 
of starch in the egg cell seem to be used up in the course of its 
activities. 
After the formation of the embryo a number of the “nutritive 
vacuoles” still retained their normal appearance; even when the 
suspensors were so big as to carry the embryo to the very middle 
of the endosperm, they were to be observed intact, sometimes also 
witli the starch grains in them. At this time there were but few 
starch grains in the suspensors themselves, and their cell walls (like 
that of the egg) contained amyloid, for in a fresh condition they 
stained bluish with iodine. 
With the formation of the embryo, starch began to collect in 
the cells of the endosperm at the base of the egg cell and in the 
cells surrounding the embryo, but the endosperm as a whole did 
not get filled tili very late. 
The origin of the protein grains in Pinus as in the Cycads 
and Ginkgo is to be looked for in some forms of soluble protein 
compounds such as amides or liexonbases, which pass in through 
the endosperm from cell to cell. Whether or not the nucleoli of 
the jacket cells play an important part in working up the protein 
before its entry to the egg cell as suggested by Ferguson is 
difficult to say just' yet. It does not appear to us to be quite 
reasonable to look for the sole supply of protein nourishment for 
the egg to the nuclei of the jacket cells; nor is Ferguson’s 1 ) 
view that secondary nucleoli develop in the egg to be the “nutritive 
spheres” to be easily accepted. Arnoldi’s extraordinary results 
can be explained as suggested by Strasburg er 2 ) as due to 
q Ferguson, M. C., „Contrib. to the know. of the life hist, of Pinus". 
(Proc. "Washington Acad. of Sei. Vol. VI. 1904 see p. 107.) 
3 ) Strasburg er, E., „Ueber Plasmaverbindungen pflanzlicher Zellen“ 
(Jahrb. f. wiss. Bot. Vol. 36. 1901. p. 493—606. see p. 550—552.) 
