Stop es and Fujii, The nutritive relations of the surrounding tissues etc. 23 
10. So far as we could see, the granulär contents of the 
nutritive vacuoles have no nuclein among their constituents as in 
most cases they clo not stain with acetic metliyl green. 
11. The Chemical nature of the nucleoli and protein grains 
is found to be different, as shewn by the results of digestion 
experiments, supporting our view of their different origin. 
12. It is proved that the „Hofmeister Körperchen“ are not 
nuclei, and we think it unlikely they are intimately connected with 
nuclei. We suggest that they may he digestive vacuoles comparable 
in origin and function with the digestive vacuoles of lower organisms, 
which are formed as required round the temporarily deposited food 
in the egg cytoplasm. 
In the course of these investigations we have worked in the 
botanical laboratories of the Universities of Manchester, London 
and München, and the alpine Laboratory of Mount Schachen; and 
we cannot express too strongly our best tlianks to Professors Weiss, 
Oliver, and Goebel for innumerable helpful kindnesses. We are 
also indebtecl to many people for material which has been invaluable; 
in particular we must thank M r Moore of Dublin, Prof. Chamberlain 
of Chicago, Dr. Miyake of Kyoto and Drs. Yabe and Hattori 
of Tokyo. 
Manchester and Tokyo. 
Explanation of plate. 
All figures drawn with the aid of a camera lucida. hör highest magni- 
fication Beck's in oil immersion and Zeiss compens. ocular 12 were used. 
Fig. 1 . Encephalartos Lelimanni. Portion of the thick pitted wall between 
egg cell and jacket cells, shewing final pit closing lamella perforated 
only by Plasmodesmen. From hand section swollen with sulphuric acid. 
x 2200. 
w thickened wall. 
I lamella closing pit perforated by groups of plasmodesmen, cf. 
arrows a diag. 4. 
j contents of jacket cells. 
e contents of egg cell. 
Fig. 2. Encephalartos Lelimanni. Same as fig. 1. Shewing plasmodesmen 
tkrougk the thicker portions of the pit closing membrane. 
t thicker portion of membrane cf. arrows b diag. 4. 
Fig. 3. Zamia muricata. Surface view of a small portion of the thick wall 
between egg and jacket cells shewing groups of pitted areas. From 
microtome section tangential to egg cell. Stained triple. X 900. 
g group of pits. cf. diag. 4. pit to Ist degree. 
io thickest portion of wall. cf. diag. 4. A. 
io' irregularly thickened portions. cf. diag. 4. B. 
r radial walls of jacket cells. 
