6 Stop es and Fujii, The nutritive relations of the surrounding tissues etc. 
quantities in the endosperm. That the cells of the endosperm were 
rieh in sugar was established by heating drops of sap obtained 
from them by means of capillary tubes, with an aqneous solntion 
of acetate of phenylhydrazin after the method of one of us 1 ), or 
by heating sections of the tissues with the same reagent in small 
open tubes. On cooling, the cliaracteristic crystals of gluc-ozozone 
developed in large quantities, proving that there must have been 
much glucose in the tissues. Gaue sugar did not seem to be 
present to any recognisable extent. To test this, sections were 
placed in a strong aqueous solution of Invertin for l 1 }* hours and 
then tested with acetate of phenylhydrazin, but there was no 
noticeable increase in the quantity of ozozone crystals formed; 
while in the control experiments carried on at the same time with 
a weak solution of cane sugar with the addition of invertin even 
after 10 minutes we got very well marked formation of glucozozone 
crystals. 
Oxydases were present in the vascular bundles of the Integu¬ 
ments, apparently in the phloem, perhaps coinciding with the 
‘‘Leptomin” had been found by ßaciborski 2 ) in this position 
in many plants. In the jacket cells it appeared also to be 
pesent, but was difficult to demonstrate exactly owing to the 
presence of the already mentioned reducing substance in the 
surrounding endosperm cells which we found hindered the reaction 
of guajac resin. 
In other materials of an allied species of Ceratozamia just a 
little older than the above, the same faets held good, except that 
the carbohydrates were not only present in a soluble form (sugars) 
but also had begun to be deposited in the form of small starch 
grains in the endosperm cells towards the base of the Archegonia. 
Protein substance also appeared to be suppliecl in a soluble 
form at this stage, the nucellus showed very strong proteimreactions 
withMillon’s reagent, lodine (which was applied in alcoholic solution 
to sections previously treated with absolute alcohol to prevent the 
reaction of starch) and biuret reaction. In the general endosperm 
cells the reaction was faint though definite, while in the jacket 
cells and their nuclei the reactions were rnore apparent. In this 
stage we could not observe any definite granules such as were 
readily detectable in the later stages when protein storage begins 
in the endosperm. In many Cycads tlie deposition of protein 
substance in solid form appears to lag beliind that of the starch 
in and near the egg, but this seems to be reversed in the case 
ot Ginkgo where the well developed protein grains appear very 
early in the egg cell. 
J ) Fujii, K., „Kleinere Beiträge z. Mikrotechnik“. „An. d. Glaskapillar 
z. mikrochem. Analyse“. (Compte Rendu d. sean. d. 6 Congres intern, d. Zool. 
(Berne 1904). Geneve 1905. p. 531.) 
2 ) Baciborski, M., „Ein Inhaltskörper d. Leptoms“. (Berichte d. D. Bot. 
Ges. Bd. XVI. 1898. p. 52—63.) „Weitere Mitteil. ü. d. Leptomin“. (loc. 
cit. p. 119 and 123.) „Einige Demonstrationsversuche mit Leptomin“. (Flora. 
Bd. 85. 1898. p. 362' to 367.) 
