34 Olive. Mitotic division of the nuclei of tlie Cyanophyceae. 
1 t- 
is again callecl, liowever, to tlie historical table on p. 2, wliicli 
presents a most interesting variety of conclusions concerning 
fliese inclusions. But I wish to record here some differences of 
opinion as to the position in the cell of the granules. Bütschli 
(90) and others thonght that the sinne globules were chromatin 
granules and natnrally believed that they occured within the 
central body; althougli, in some higher plants, Bütschli fonnd 
them scattered through the wliole protoplasm. Kohl (03), pro- 
bably because he had not examined the matter in sections, 
agrees that they occur only in the nucleus. He gier says, on 
tlie other hand, that tlie „slime vacuoles“, as he calls them, as 
well as the cyanophycin granules, lie only in tlie cytoplasm, 
outside the nuclens. Fig. 27 is a cross section of Oscillatoria , 
showing a large slime globnle, obviously imbedded in one side 
of the nucleus. Fig. 8 represents a preparation, likewise stained 
with iron liaematoxylin, in which the slime globules are stained 
more or less dark. In this hgure, one such body is shown, 
pressed into one side of each nuclens. On the other hand, 
certain minute globules in hgs. 7, 11 and 13 seem to be actually 
enclosed within the limits of tlie central body. Therefore, while 
it appears possible, since there is no nuclear membrane, that tlie 
slime globules may be sometimes, entirely enclosed by the sub- 
stance of the central body, they lie usually in tlie cytoplasm in 
the immediate vicinity of the nucleus. 
The cyanophycin granules may sonietimes be seen with high 
powers in living plants of Oscillatoria and other forms (fig. 1). 
Tlieir relative position is much better shown, liowever, in sections 
which have been stained with eosin, e. g., together with some 
other differentiating stain to bring out the nucleus and other 
parts of the cell. Fig. 21 is such a preparation of an unde-~ 
termined species of Oscillatoria and hg. 22, of the minute species, 
0. splendens. In both cases, the cyanophycin granules lie in 
the cytoplasm, in close proximity to the cross walls. In one 
cell in hg. 21 is shown a minute refractive crystal, lying in a 
vacuole, wliere, normally, the cyanophycin occurs. In hg. 23, 
the granules are shown in a cross section of Oscillatoria, Fig. 48 
represents a preparation of Calotlirix in which the granules are 
stained with safranin, and hg. 56, of Nostoc , similarly pre- 
par^d. In these last drawings, as well as in one of Cylindro- 
spermum (hg. 77), we observe that the granules occur, scattered 
irregularly, throughout tlie cytoplasm. 
Experiments witli digestion. 
Some experiments were undertaken by the writer in Order 
primarily to discover whetlier the assertions of Fischer (97) 
are correct in regard to certain conclusions of Zacharias (87) 
and Bütschli (90). Zacharias claimed that pepsin partly 
digested the peripheral cytoplasm in Oscillatoria , leaving tlie 
undigested chromatin of the central part as granulär, refractive 
