322 
As o, On tlieFature of Oxidases. 
From tlie above results, I became convinced tbat amido- 
compounds decompose nitrite in a very faint acid solution and 
it is necessary to make tlie solution alkaline to preserve the ni¬ 
trite. Hence l made analogous experiments with plantjuices. 
Experiment with Sagittaria. 
18 grams of tlie buds of Sagittaria were cruslied, extracted 
with 100 c. c. water and divided into three ecpial parts. To 
one a few drops of acetic acid, to the other a few drops of 
caustic potash were added while the third served as control. 
Fach solution was heated to boiling for live minutes and tested 
as mentioned before: 
Control 
Alkaline 
solution 
Acid 
solution 
P ot as siumio did- 
starch reaction 
Distinct 
Distinct 
No reaction even 
after several 
liours 
G r i e s s reaction 
Guaiac reaction 
, V) 
~No reaction 
at all 
77 
No reaction 
at all 
faint 
No reaction 
at all. 
Quite similar a test was repeated, but in this case, the So¬ 
lutions were heated to 95 0 C for 10 minutes and filtered after 
acidification with acetic acid, which had produced some pre- 
cipitate: 
- 
Control 
Alkaline 
solution 
Acid 
solution 
P otassiumio did- 
starch reaction 
No reaction at 
first, but after 
10min. appeared 
gradually 
Distinctly 
at once 
No reaction at first, 
but after 10 min., 
a reaction appeared 
although weaker tka n 
in the control case 
Griess reaction 
Distinctly 
Distinctly 
Distincly 
Guaiac reaction 
No reaction at all 
No reaction 
at all 
No reaction 
at all. 
solutions were filtered and tlie filtrates were tested witli tlie reagents ab- 
ove-nieiitioned. Hereupon Glriess and iodine reaction appeaied \eiw dis- 
tinctly, thongli tlie latter was a little weaker in boiled liqnid than in tlie 
control; but no blue reaction of guaiac at all appeared. Similar facts were 
observed repeatedly. 
