As o, On tlie Nature of Oxidases. 
325 
cliloricle. The Griess reaction appeared, but only in traces. The 
main part of the jnice (about 25 c. c.) was mixed with tliree 
times of its volume of alcoliol (90 ° 0 ) and filtered. The filtrate 
showed a very fahrt Griess reaction, but no reaction for oxidi- 
zing enzyms and also with potassium-iodid- starch, wliile the 
aqueous solution of tlie well-waslied precipitate gave very strong 
reactions for oxidizing enzyms, but not the G-riess reaction and 
also no iodine reaction. 
Experiment with Pea. 
4 grants of the root of a full grown peaplant were crushed 
and extracted with 30 c. c. wather. The juice obtained had al¬ 
most a neutral reaction and gave strong reactions for oxidizing 
enzyms, but neither liberation of jodine nor the Griess reaction. 
20 grants of the green pärts ] ) of the pea plants were crushed 
and extracted with 30 c. c. water, and tested in a similar wav 
/ i. 
as above with the same results. 4 grants of the root-tubercles 
were now crushed, and the pressed juice of a faint acid reaction 
gave strong reactions for oxidizing enzyms. but no liberation of 
iodine nor the diphenylamine reaction. Also Fehlings solution 
was not reduced. Further 5 grams of the white part of the 
stein of pea-shoots (5—10 cm. long) were crushed and extracted 
with 2 c. c. water. After filtering and adding a few drops of 
concentrated solution of basic lead acetate, the filtrate was mixed 
with some sulphanilic acid and sulphuric acid and filtered. To 
the filtrate, a few drops of c-naphthylamine hydrochloride were 
added. whereupon the Griess reaction appeared feebly, but dis- 
tinctly. Again, 10 grams of the white part of the pea-stem 
were crushed and the juice was pressed out. The juice produced 
strong color reactions for oxidizing enzyms and diphenylamine 
reaction for nitrate, but neither Griess nor potassium-iodid-starch 
reaction for nitrite. The juice was now treated with a few drops 
of concentrated solution of basic lead acetate and filtered To 
one part of the filtrate, some sulphanilic acid and sulphuric acid 
were added and again filtered. The filtrate showed here again 
a distinct Griess reaction upon the addition af c-naphthylamine 
hydrochloride. With 10 grams of the green part of tlie same 
shoots, were, after the same treatment, tested in the sanier man- 
ner. But neither the diphenylamine reaction nor the Griess re¬ 
action was here obtained. 
16 grams of Sagittaria shoots (of green color) were crus¬ 
hed with 50 c. c. water and filtered. The filtrate showed very 
strong reactions for several oxidizing enzyms, but a very weak 
potassium-iodid-starch reaction. * 2 ) This filtrate was diluted with 
0 In tis case, it is necessary to add nracli guaiac tincture; otlierwise 
tlie blue color soon fades out. 
2 ) After boliing aud filtering, tfie filtrate skowed a weak iodine reaction, 
but no reaction for oxidizing enzyms. 
