Newcombe, Sensitive Life of Asparagus plumosus. 
29 
The tips of 3 of these 14 seedlings, for a length of about 
3 nun, were declined at a smaller angle than the part lower down 
when the experiment ended; but in the rest of the plants, the tip 
continued at the same declination as the part below. 
These preparations were continued for 7 weeks after the 
shoots began to appear above ground, for 34 days after the first 
seedling took its final diageotropic position, and 28 days after the 
last seedling took its final diageotropic position. These shoots 
haye stopped growing; there is no indication of a resumption of 
the vertical position. 
C. Behavior of twining Shoots. 
Ä description of the appearance and behavior of a normal 
twining shoot is given under the “Introduction“ to this paper. 
Several shoots growing in free earth in a bed in a green- 
house were chosen for experiment; all showed that they were, in 
ordinary conditions, destined to climb. They were tested for their 
behavior in darkness. These shoots were covered with sheetiron 
or cardboard cylinders 20 cm in diameter and of various hights. 
1) The first shoot selected was 4 mm diameter at the ground, 
and 27.5 cm in hight. It was orthotropic and bore no appendages 
except the nodal scales. Five days after exclusion of the light, 
the shoot had reached a hight of 39 cm, and its tip diverged some 
45° froin the narrow bamboo stäke stuck into the ground and 
rising in the axis of the cylinder. It showed no evidence of 
twining. The tip continued to rise in the same direction for 3 
days more when it arched over, forming an arc of more than a 
semi-circle. Such a form of tip as this is taken in the normally 
growing plant as evidence of twining; but this tip did not twine. 
For two davs more this shoot was watched. and then it died from 
over-heating in the cylinder unprotected from direct sunlight. The 
shoot had reached a hight of 41.5 cm but had not twined about 
the stäke. It is probable that, had it been growing in the light, 
it would have twined for the last 2 of the 10 days it was under 
experiment. 
In subsequent experiments like the foregoing the covering 
cylinders were wrapped with several layers of wiiite paper, air 
spaces being left between the layers. The removable caps, too. 
covering the upper ends of the cylinders were similarly protected 
from the sun, and there was no farther trouble from over-heating. 
The interior of the cylinders was thus kept cooler than the outside 
air in the hottest part of the day. This study was made thru the 
month of August. 
2) A second shoot 35 cm in length, 4 mm diameter at the 
ground, was covered by a cylinder. Its tip at the time of covering 
had an S-shape curve 8 cm long. The shoot was tied to a bamboo 
stäke about 7 mm in diameter. After 2 days under cover, the 
tip became vertical. On the 5 th day in the dark, the shoot had 
grown to a length of 58.5 cm, and its tip was bent in an arc of 
