August, ’23] 
WILLARD: GERMINATION OF SEEDS 
391 
Dent corn was not affected by any treatment used. The other seeds 
were more or less injured, the injury increasing with the length of ex¬ 
posure and the size of dose, though not in any definite ratio. 
However, the seeds were more injured by the bisulphide than appears 
in these tables. The first effect of the treatment was to delay germina¬ 
tion one or more days later than the check. The delay was due to a 
definite injury to the seeds as is shown by the fact that a treated sample 
showed the same delayed germination a year after the treatment. A 
few examples from the large amount of data secured are given in Table 6. 
Table 6. Data to Show Retardation 
Seed and Treatment Number germinated 
2 
3 
4 
5 
6 
7 
8 Total 
days after test was started 
Timothy, check. 
60 
30 
7 
97 
Timothy, 1.6 cc. for 21 ‘days. 
0 
21 
66 
87 
Buckwheat, check. 
91 
2 
93 
Buckwheat, 24 days, 3.2 cc. 
66 
25 
2 
93 
Buckwheat, 24 days, Saturated atm. 
14 
20 
0 
8 
16 
13 71 
Wheat, check. 
.77 
22 
99 
Wheat, 16 days, .2 cc. 
.78 
19 
97 
Wheat, 16 days, .4 cc. 
.46 
53 
99 
Wheat, 16 days, .8 cc. 
.56 
44 
100 
Wheat, 16 days, 1.6 cc. 
.21 
74 
4 
99 
Wheat, 16 days, 3.2 cc. 
.19 
84 
7 
93 
Wheat, 16 days, Saturated atm. 
. 4 
34 
32 
3 
73 
In the wheat series the sample treated with .8 cc. and 1.6 cc. both made 
a total germination equal to the check yet they were definitely injured 
by the treatment as shown by the delayed germination. In the timothy 
series on the third day it appeared that the treated seed was entirely 
killed. Finally there was only ten per cent difference between the check 
and the treated sample. Whether the subsequent development of 
these slow germinating seeds would be normal has not been determined. 
The seedlings appeared perfectly normal on the blotters. 
Table 7. Seeds Immersed in Liquid CS 2 for Various Periods 
Check Treated 
Corn, 24 hours.100 100 
Corn, top of kernel cut off, 24 hours.100 100 
Corn, top of kernel cut off, 8 days.100 86 
Corn, punctured over scutellum, 24 hours.100 88 
Corn, 10 days.100 92 
Corn, 30 days.100 88 
Wheat 2 hours. 97 79 
Wheat, 20 hours. 97 44 
Wheat, 43 hours. 97 34 
Oats, 24 hours. 95 65 
Soybeans, 24 hours. 86 70 
