110 
REPORT OF BOARD OF FISH AND GAME COMMISSIONERS. 
a ropy, thick, white appearance, did not mix with water, but sank to 
bottom when put in clear water or normal salt solution. Eggs were 
taken from this lot, and milt, which had been caught in a vial of normal 
salt solution, added; the washing was done in distilled water, and they 
were placed in distilled water to swell, but did not swell at all. 
April 29 .—Eggs were taken at intervals and examined under the 
microscope, but no sign of segmentation could be found. Eggs were 
examined under high power of microscope for bacteria and fungus, but 
none could be distinguished. The eggs are constantly turning while 
floating to the top of jar and going over; an examination of these eggs 
shows that the germinal disc was broken or pinched off, and in some 
cases the yolk was ruptured. At 6.30 p. m. (twenty-four hours), fully 
50 per cent of the eggs had passed off this way. 
April 30, 7.00 a. m .—An examination of the jars showed that very 
few eggs were left, and these showed no signs of segmentation, hence 
jars were emptied. Specimens in mould dish were examined at 9.00 
a. m. for bacteria and fungus, but none found. Examined again at 
4.00 p. m., when it was found the bacteria (Bacterium terms) had 
developed in countless numbers. A distinct odor of putrid fish was 
discerned on opening dish. 
4.00 p. m .—A number of males and females were examined on wharf, 
but no ripe ones found. Milt from these males showed no movements 
under microscope in three to five minutes. 
Lot “B,” April 30, 7.00 p. m. 
This lot of eggs was from a female which had been dead about one 
hour, and male had been dead about the same length of time. Both 
eggs and milt were taken at wharf. Eggs taken in a new dry tin pan. 
The milt expressed directly over them. The pan dipped immediately 
(in about fifteen minutes) into river from side of boat. They were in 
this water with the milt while carried to the hatchery (about three 
minutes), and washed in water from overflow taken in the hatchery. 
Allowed to swell one hour before beginning to put in jars. The last 
of them remained in pan 2^ hours. About 25 per cent of this lot 
turned white before they were put in jars. A study of these eggs 
showed that no impregnation took place, just as in lot “A,” The eggs 
swell, the germinal disc contracts and forms a cap on one side of the 
yolk. This cap remains in position up to about twelve hours, when 
it pinches off. The egg turns a whitish color, becomes buoyant and 
floats off through the siphon tube. A part of the eggs in this lot were 
taken in normal salt solution and milt, which was caught in normal 
salt solution applied. Specimens from these were preserved, and a 
study of them shows that they acted in just the same way as the others. 
