APPENDIX 
111 
Lot “C.” 
Tried the experiment of taking some of this lot of eggs without milt 
and swelling them, placing them in a jar under same conditions as 
the impregnated eggs. They developed exactly as lots “A” and “B,” 
and the germinal disc pinched off in just the same way. This is prac¬ 
tical proof that the eggs will develop to this stage without impregnation, 
and also that impregnation did not occur in lots “A” and “B.” 
Lot “C,” May 6. 
Male and female alive and in prime condition. The dry method was 
followed. The self-straining bucket was used. This consists of a 
cylinder of perforated tin, fitting inside the regular collecting bucket, 
barring a space of two inches between tin and side of bucket. Water 
is poured over the eggs, and the movement of cylinder in the bucket 
strains off the milt. The first segmentation occurred in two hours, and 
it was estimated that 30 per cent of them would develop embryos, and 
25 per cent of them did develop into fish. There was about 50 per cent 
died after developing into embryos, thought to be smothered from lack 
of circulation of water, due to collection of sediment on sides of jar. 
The non-fertile and unimpregnated eggs passed over within twenty- 
four hours, just as in the first lots “A” and “B.” A part of these 
eggs was saved and left in the jar to watch development of fungus 
and bacteria. 
May 8 .—The first trace of fungus was found on the dead eggs in the 
above jar within forty-eight hours after taking; specimens were removed 
to mould for study. 
May 9 .—The fungus consists of two species. One has the appearance 
of saprolegnia, the other a segmented form of a higher order, and 
both are in fruiting stage, showing that they develop very rapidly. 
So far no trace of the fungus on live eggs. In the case of the ripe 
eggs taken from spawned female, a small percentage of fertilization 
was obtained in a normal salt solution after eggs had been taken three 
hours. 
Lot “D.” 
May 24 .—Eggs from two females taken by McLeod. Milt from buck 
taken by McLeod at same time. Wet method employed by McLeod. 
Brought into hatchery half an hour after taking, with milt still on. 
Washed up in hatchery in four dish pans about 1,000,000 eggs. Appar¬ 
ently 30 per cent of segmentation at two hours. Apparently 25 per 
cent had turned white. 
May 25 .—Eggs disturbed at twenty-four hours by Ball, condensing 
the good eggs into three jars and pouring off bad ones. 
May 26 .—Do not seem to have suffered by handling. 
May. —Twenty-five per cent of this lot hatched into fish. 
