112 
REPORT OF BOARD OF FISH AND GAME COMMISSIONERS. 
Lot “E.” 
May 24, 8.00 p. m .—Eggs taken by McCrea from female which he 
thought was ripe. Milt from buck which he said was in prime condi¬ 
tion. Dry method employed. Brought to laboratory in spawning pan. 
"Was very white with milt which was stringy (not ripe). Washed up 
in hatchery. At two hours, about 1 per cent segmentation. Put in 
jars and kept twenty-four hours. 
May 25 .—No sign of embryos; hence thrown away. 
Lot “F.” 
May 24, 9.00 p. m .—Eggs from spawned out female taken by “Nib- 
sey, ” about 3 miles above Bouldin. Milt from male taken at same time. 
Wet method employed by Ball. Female had been very roughly handled 
in taking from net, but eggs had ripe appearance. Eggs taken in 3 
gallons of water (about 20,000). Milt added and left for one hour; 
at two hours—50 per cent segmentation. 
May 26 .—About 50 per cent ready to hatch. Used for experiments 
with copper sulphate. 
Lot “G 
May 25 .—Eggs from a “spawned” female. Milt from buck taken 
half an hour later (not ripe). Wet method employed by McLaughlin. 
Brought into hatchery in self-straining bucket. In two hours about 
50 per cent fertilization. Put in open jar. 
Lot “H ” 
May 25 .—Eggs from female taken by Woods. Milt from buck taken 
by Woods. Female in good condition and eggs apparently ripe. Male 
small and milt green (stringy). Wet method employed by Cassell. 
At two hours about 25 per cent fertilization; hence were not put in jars. 
Lot “I.” 
May 28 .—Female and male taken by McLeod. Eggs apparently ripe. 
Milt not very good. On questioning them closely, found they had taken 
the female from net, expressed the eggs in pan dry, added a little water 
to keep them moist and kept them in the water until they got a buck 
(about fifteen minutes), then added the milt. The eggs thus had time 
to begin swelling before milt was added. At two hours about 5 per 
cent of fertilization. 
Lot “J.” 
May 28 .—Female and male caught by Herman Wredt, about 3 miles 
up river from Bouldin. Said they were both ripe. Wet method 
employed. Eggs kept in gasoline can for two hours. Washed up in 
hatchery. Developed 25 per cent of fertilized eggs. 
Lot “K.” The Banner Lot. 
May 28 .—Female and male taken by Woods. Both in prime condi¬ 
tion. (Eggs and milt ran freely.) Eggs a golden green color when 
