APPENDIX. 
113 
taken. Milt white, but ran out like cream. Eggs taken in spawning 
pan, a little water added, and a very small quantity of milt added. 
The water was changed frequently in transporting eggs, as they were 
carried in boat for five hours. These eggs were taken in Disappoint¬ 
ment Slough, some 14 miles from hatchery, and in the night-time. He 
had no bucket in which to place the eggs, so transferred them from 
spawning pan to a stew kettle, which he happened to have. In working 
with their engine, he would let eggs go for an hour or more at a time 
without washing. It was between five and six hours from the time 
eggs were taken to time they reached the hatchery. He washed them 
on arriving at his ark, and when they were brought into hatchery not 
a particle of milt could be seen on them, and there were very few 
dead eggs. All had a clear greenish color. There appeared to be 85 
per cent of them fertilized when they were brought in. 
May 30 .—There were actually hatched from this lot out of 280,000, 
227,000 fry, or 71 per cent, and this number planted in shallow water 
about 3 miles above Bouldin. 
Lot “L.” 
May 28 .—Male and female taken by “Nibsey. ” Spawning and 
fertilization attended to by Grey. He thought these eggs ripe, but 
sample brought to the laboratory showed very dark green with white 
spots. He said fish were very roughly handled in taking from the net. 
In two hours about 5 per cent fertilization. Put up and kept twelve 
hours (no good eggs). 
May 29 .—Male and female taken by Woods. Eggs and milt taken 
by McLaughlin. Found a few eggs (about half pint) left in female 
when she was brought in by Woods. They were a light yellowish green, 
ran freely, and separated easily in water. The milt was examined and 
found to be not very lively; it came out in thick lumps. Wet method 
used. Eggs brought to hatchery in thirty minutes after taking. 
Washed in laboratory. Developed 50 per cent of fertilized eggs. 
GENERAL CONDITIONS, TEMPERATURE OF WATER, ETC. 
On Saturday and Sunday it was warmer than at any time this spring, 
bringing the water up to 66 degrees or 68, which brought a fair run of 
bass on Sunday afternoon and with varying luck during the week. The 
north wind seemed to stop the catching of bass, though a few other fish 
were caught. The sudden drop of the temperature on Thursday night, 
combined with a northwest wind, stopped the bass entirely, so that the 
first drift on Sunday, May 31st, yielded only one or two very small 
bass, and the second drift, Sunday night, only a few green males. 
EXPERIMENTS WITH THE SPERMATOZOA. 
Spermatozoa are active for about three minutes in water, after which 
time their swimming motion ceases. Placed on a microscopic slide they 
8—FC 
