APPENDIX. 
117 
dead eggs and attach themselves to the good ones, one bad one sometimes 
having two or three good ones attached. 
It was found necessary to turn the water off during this stage, flushing 
off the dead eggs occasionally in order to not smother the live ones. 
One of the jars containing the larger percentage of dead eggs and in 
which the carrying over was greatest, was treated with copper sulphate 
(1-100,000) twice (30-minute interval). The water was then turned 
on in a stronger current than usual. This jar was kept going all the 
time that the other jars were shut off. The dead eggs moved off slowly, 
and while the live ones were in constant motion they did not go out. 
From this experiment it would seem that the fungus and micro¬ 
organisms were killed by the application of the copper sulphate, and 
when killed were removed from the eggs by the constant motion of the 
eggs. The dead eggs were thus carried off without their becoming 
attached to the live ones, and the necessity of turning off the water 
(which endangers the fish from lack of oxygen) to keep in the good eggs 
was avoided. 
