See Ji. thin [illegible]
e I
Impregnation of queens. The three queens dissected by Dr. Leidy prove the existence
of a spermatheca. Retarded impregnation, the great mystery now. (1st) Let wings be taken
from a young queen before impregnation, see if she will lay. (2) Examine young queens to see before
impregnation the condition of eggs in their ovaries. May they not lay as hens without the male. (3)
Keep young queen in small wire box in the hive accessible to bees, with drone and worker
comb, see if any eggs laid. (4) Examine retarded queens to see if they are
impregnated. (5) Touch drone eggs, leave them in cells, put some of the worms a few
days old into worker cells, try to put eggs also. Touch eggs of retarded queens. 
Worker changed to queen by larger cell, etc. why not drone to worker by impregnation. 
February 18, 1861. Entice, by feeding liquid honey, a stock
Impregnation of Italian having Italian drones, to fly an hour or two
Queens earlier than the common drones. Fasten
with thread (See p. 127 of my book) an Italian
queen a few days old, and let her fly in front of this
hive. (She should have had her entrance to hive so small
that she could not have got out for impregnation). The
string might be so short that we could see the drone and
pick him up to know for certain that he was an Italian. 
By closing entrance of hive when drones out (See Mr. Cary's observations
on impregnation) we might keep the queen close to the hive
and thus secure our object even if other drones were on
the wing. Advantages. 1st. Would not lose so many young
queens. 2nd. Be sure that our queens are impregnated by
Italian drones, even if we or our neighbors have black
drones. 3rd. Cannot only breed queens from our best
Italian queens, but can have them impregnated by
drones from a queen producing the handsomest drones. 
No other plan can make us sure until a long
period after impregnation, that our queens have been
impregnated by Italian drones. (over)