Mailing bees, see journal volume 28, 1879
Mater Regia Huber Maintains that without fecundation there
Queen. will be no eggs laid by the Queen. 
Dzierzon, that Queens can lay drone eggs
without fecundation. Huber asserts that if impregnation be retarded
for a certain time, the Queen can only lay drone eggs. 
May not such a seminal vesicle of the Queen from some cause
be incapable of being filled. If fecundation be delayed, beyond a
certain time, this can be established by dissection. In this
way both these writers will be substantially reconciled, with this exception
that Huber's opinion that fecundation is required to lay the
eggs of drones would not stand, the facts as detailed in his observation
are not inconsistent with my supposition. Dzierzon himself maintains
that unfecundated dated Queens very rarely lay any eggs at all. 
Experiment. By my [illegible] several swarms may be made to occupy the
same hive. After they have been in the hive for some time, they may be allowed to intercommunicate
without any risk of quarreling. We may thus determine whether two or
more Queens will live in the same hive where the combs are not kept quite distinct. 
Drone eggs. It is certain that the Queen knows what sex the eggs will produce before she lays
them. If she can know this, is it more difficult to suppose that she knows it because of the way
in which she chooses to extrude them. This would make it easier for her to know. We know that in the
aphids one impregnation will last for several successive generations. The same facts have lately been
observed in other [crossed out: anim] creatures. The egg that produces a Queen is impregnated. The virtue of this impregnation
may affect the way of the bee so that her eggs may have vitality. And yet an impregnation of this bee may
be necessary to enable her to produce young as highly engaged as workers. This could account for fertile
workers. This can be determined by careful experiments. (1) Let a number of hives be deprived of
Queens. When Royal brood sealed, take all but one, put her by gauze wire so that she cannot
get to bees, take off her wings, see if any eggs laid, and what kind. After trying
restore perfect Queen. (2) In observing hive with Movable frames watch for Queen laying eggs in drone
cells, remove, touch part of them with pencil dipped in semen, restore. (3) Do same with eggs of a
Queen when impregnation has been retarded. (4) Same with unimpregnated queen laying drone eggs.