Experiment with Mr Kramers process for [illegible] 
fertilization. Tuesday June 21st 1881 got from Mr [illegible] 
a nucleus with sealed [illegible] Queens (two) and a few eggs 
and larvae from his old [illegible] Queen also a [illegible] both sealed 
[illegible] from one of her daughters. 24th 5 AM a good Queen 
hatched. Made a good nucleus in lower story of [illegible] hive 
almost all the old bees to return home, and at 7 PM, gave them 
[illegible] Queen and shut them up. 25th 5 AM Queen all right 
gave water, admitted light at 1 PM, many bees flew, [illegible] the 
honey board etc allowed most of those bees to escape as I thought they 
[illegible] been out. 27th very few took wing when light was given 
today. Weather very hot, ventilators at the crack between the two 
parts of honey board conditions the same as yesterday 
cannot see that the bees suffer at all [illegible] 
uneasy at being confined, young bees are hatching, the 
sealed brood is [illegible] covered, not a single drone or drone larvae 
in the hive. My [illegible] drones have not yet hatched. As 
there are no [illegible] drones anywhere for miles if this Queen 
becomes purely mated from drones given to her nucleus the demonstration 
that she mated in the box will be complete. As I shall 
have the exact time of hatching of [illegible] drones it will be easy to 
determine how soon after hatching a drone can mate. Good 
[illegible] about as long as a Queen [illegible] if the bees bear 
confinement well can remove Queen as soon as I see on her 
[illegible] that she has mated, and give them another. If an Italian 
Queen is mated to one of the confined drones the progeny [illegible] 
[illegible] another [illegible] 




