200 
Vol. XXIV, No. 3 
Journal of Agricultural Research 
Many other series of inoculations were made with tissue from Pythia- 
cystis gummosis lesions in different localities, varying widely in climatic 
and soil conditions, with the same general results as to type and progress 
of the disease. Inoculated trees contracted the disease just as readily 
on light sandy soils in orchards in which Pythiacystis gummosis had never 
occurred before as in orchards on heavy soils in which the disease had 
previously prevailed (PI. i, E, P). Variations in the development of the 
disease in individual trees and at various seasons of the year are indicated 
in Table II, which summarizes some of the inoculations with tissue from 
diseased lesions. 
Tabi<E II .—Inoculations on trunks of i 6 -year-old lemon trees at Chula Vista, with 
tissue from diseased lesions ^ 
Experiment No. 
Date of inocula¬ 
tion. 
Date of observa¬ 
tion. 
Size of area .2 
Gum formation,^ 
Cm. 
(Mar. 
24,1912 
—. 
3 
Aug. 
2,1912 
25 by IS 
3 (PI- I. A.) 
Feb. 27,1912 « 
Sept. 
19,1912 
30 by 30 
3 (PI- I, B.) 
j. 
Nov. 
15,1912 
33 by 30 
I 
Mar. 
II, 1913 
48 by 80 
— 
May 
24,1913 
—. 
I (PI. I, V.) 
Sept. 
3,1913 
— 
3 (PI. I, C.) 
'Nov. 
15,1912 
— 
3 (PI- I, F-) 
Mar. 
II,1913 
20 by II 
3 
May 
24,1913 
38 by II 
3 
2 . 
Sept. 21,1912 < 
July 
9,1913 
60 by 13 
— 
Sept. 
3,1913 
66 by 15 
— 
Oct. 
31,1913 
68 by 16. 5 
2 
Apr. 
18,1914 
75 by 16.5 
— 
'Nov. 
IS, 1912 
— 
3 (PI- I, E.) 
May 
24,1913 
49 by 22 
2 
T. 
.do. 
July 
9,1913 
60 by 22 
3 
Sept. 
3,1913 
66 by 22 
— 
Oct. 
31,1913 
70 by 22 
— 
Apr. 
19,1914 
84 by 22 
— 
Feb. 
6,1913 
— 
3 
Mar. 
11,1913 
13 by 8 
— 
A . 
Nov. 16,1912 
May 
July 
24,1913 
29 by 13 
46 by 33 
2 
9,1913 
3 
Feb. 
6,1913 
— 
0 
[Mar. 
II,1913 
7- 5 by 5 
I 
May 
24, 1913 
16. 5 by 5 
3 
■c . 
.do. 
July 
Sept. 
9,1913 
16. 5 by 5 
21. 5 by 7. 5 
- (PI. 7, A.) 
3,1913 
Oct. 
31,1913 
23 by 10 
3 
1 All experiments except No. i were made on trees growing in light sandy soil. 
* The first number gives the greatest extension vertically and the second number the greatest extension 
horizontally. This applies also to similar data in the tables that follow. 
*o=*'none; i-*slight; 3 »»medium; 3=«copious; a dash indicates that no data were obtained. 
INOCULATIONS WITH DISEASED FRUITS 
After it seemed probable that the fungus commonly associated with 
Pythiacystis gummosis was the same as that causing brownrot of lemon 
fruits, Pythiacystis citrophthora, it was of interest to determine whether 
the disease could be induced directly from the brownrotted fruits, either 
in wounds or on uninjured tissue. 
