250 
Journal of Agricultural Research 
Vol. XXIV, Na 3 
N. viscosum is more diflScult to transmit artificially than the common 
tobacco mosaic. Datura stramonium was the only solanaceous plant found 
susceptible to both mosaic diseases. Allard suggests that results of inocu¬ 
lations with tobacco mosaic by various European investigators which are 
inconsistent with his own may be due to the existence of different types 
of mosaic in the Solanaceae. 
COMPOSITE RECORD AND RESULTS OF EXPERIMENTS 
The technic of the work here reported has been exactly the same as 
that described in a previous paper (j). All inoculations were made 
with one-half to 2 cc. of cell sap, obtained by squeezing young stalks in 
a powerful press under mineral oil. The virus or healthy sap was in¬ 
jected near the growing point by means of Leur all-glass hypodermic 
syringes provided with Yale 24-gauge needles. The results of the experi¬ 
ments are given in support of the facts presented in the preceding pages. 
In order to save space, composite records of the experiments are shown 
in tabular form. 
^able I includes tests made for artificial transmission. 
TablH l,—rCoinpqsite record of tests of the artificial transmission of grass mosaic 
Num- 
Date 
inoculated. 
Results. 
Species. 
ber of 
plants. 
Inoculum. 
Date. 
examined. 
Condition. 
Saccharuni ojSUcinaruin 
var. Louisiana Ptir- 
pl^ 
Do... 
4 
Oct. 4, X990 
.do........ 
Virus from su^ar cane 
variety B64S0. 
Sap from healthy su^ar 
Oct. x8, X930 
Nov. X 5 ,X 930 
3 mosaic. 
All healthy. 
Do. 
■A 
.do. 
variety B6450. 
Virus from B64C0 passed 
throueh Berkdeld filter. 
Virus from B6450 mixed 
with equal part of 0.3 
per cent phenol. 
Vims from B6450 mixed 
Oct. x8, X920 
3 mosaic. 
X xixbsaic. 
Do. 
A 
.....do... 
Do......... 
# 
4 
.....do........ 
Nov. IS, X920 
All healthy. 
Do....i... 
4 
.....do.... 
with equal part of 0.05 
per cent CUSO4. 
Virus from B6450 mixed 
.do. 
Do. 
Do.;............. 
.do........ 
with equal part of s per 
cent formalin. 
Vims from B6450 mixed 
with X per cent Carrol 
Dakin solution 
Virus from B6450 mixed 
with o.x per cent HgCh. 
Virus from B6450 diluted 
Do. 
Do. 
A, 
.....do..i..i.; 
.do.. 
Do. 
Do......... 
*r 
4 
.do. 
.....do.. 
Do. 
Do............... 
4 
.do.. 
x/xoo with distilled water 
Virus from B6450 diluted 
.do. 
Do. 
Do...../.......... 
, Do;....;......... 
• 4 
8 
.....do........ 
Auff. 4 .I 9 SW 
1/1,000 with distilled 
Water. 
Virus from B64S0 diluted 
1/10,000 with distilled 
water. 
Vims from l/ouisiana 
Purple. 
.....dd*,...... 
AU£ 28 ,X ^30 
Do. 
8 mosaic. 
Zea mays vat, V. S. 
Sel^on No. xSs. 
t' 
..I..do........ 
Sept. 99,1930 
Aug. 38, X930 
All healthy. 
3 mosaic. 
3 
.... -do........ 
.do.,................... 
-1 - - 
Syniherisma sam/uina- 
Its . * . 
Coix taehrynuHobi ..... 
Beiimmtum . 
Sddcharum officmahitn 
Tar. loiiisiaxia; Pur- 
6 
.dO;.. ........ .. 
.....do........ 
5 mosaic. 
9 
6 
__ ..... 
.....do........ 
__ .do... 
.....dd...._.........i... 
.... ... 
Sept, 39,1920 
Aug. 38,1930 
Sept. 29,1920 
.....do........ 
Bothhe^thy.. 
6 mosaic. 
8 
■ • 4' 
;_do...--_ 
Sap from healthy sUkar 
cane, variety I;ouisiana 
Purple. 
.... .do....... 
All healthy. 
Do. 
Hofnts sorgkum^ 4 .. . 
SjmtHerUfna sanffuhtor- 
lis, 
Coix lachryma-Jobi ., . 
Pmnis^hsifiglaumm .. 
■id.i ..U. 
<9 
, 3 
... . .do........ 
.....do.. ..... 
... . .do . 
Do. 
’ 6 
,,. .dn. 
. -do.... .. 
.do .. 
Do. 
t 
. -/ 6 
.....do.. . 
1 r ' i' • t r /i ' 
i....do . 
.do.... .. 
. do . 
.....do.. ...... 
Do. 
Do. 
