272 
Journal of Agricultural Research 
Vol. XXIV, No. 3 
The net gain of nitrogen fixed by all the cultures in the straw solution 
exceeded the net gain in the dextrose by an average of 4.13 mgm. 
The experiment was repeated, and a daily analysis of the cultures was 
made. The cultures in this case were aerated for four days. The data 
are tabulated in Table VII, 
Tabi^E VII .—Effect of straw on azofication 
Cul¬ 
ture 
No. 
Nitro¬ 
gen in 
control. 
1 day. 
2 days. 
3 days. 
4 days. 
Medium. 
Gross 
nitro¬ 
gen. 
Net 
nitro¬ 
gen. 
Gross 
nitro¬ 
gen. 
Net 
nitro¬ 
gen. 
Gross 
nitro¬ 
gen. 
Net 
nitro¬ 
gen. 
Gross 
nitro¬ 
gen. 
Net 
nitro¬ 
gen. 
10 B. 
D. A. 200 CC. ^. . 
Mgm. 
Mgm. 
Mgm. 
Mgm. 
Mgm. j 
Mgm. 
Mgm. 
Mgm. 
Mgm. 
I. 0 
4. 55 
3 - 55 
18. 46 
17.46 , 
19. 20 
18. 20 
24. 96 
23- 96 
10 B . 
D. A. S. 200CC.2. 
10. 07 
10.40 
•33 
20. 93 
9.87 
30. 29 
20. 22 
29.44 
19 - 37 
19. . . 
D. A. 200 cc. ^. . 
I. 0 
8.38 
7 * 38 
15 -14 
14.14 
24. 70 
23. 70 
25. 02 
24. 02 
19. .. 
D. A S. 200 cc.^. 
10. 07 
13- 78 
3-71 
25.02 
14 - 95 
39 - 32 
29.25 
42.83 
32. 76 
' Dextrose-Ashby 200 cc. * Dextrose-Ashby and straw 200 cc. 
In general, the results are similar to those reported in the previous 
experiment. However, culture lo B showed an approximate net gain of 
2.0 mgm. at the end of the third day in the straw culture and a loss of 
over 4^3 mgm, of nitrogen on the fourth day. An average of the two 
cultures indicates a net gain of 4.15 mgm. for the straw medium for the 
^tire period of the test. 
As expected, a greater net gain of nitrogen was recorded for the dextrose 
solution than for the straw medium for the first two days. This indicates 
that the straw is not utilized until all of the greater part of the dextrose 
is consumed. 
Calculating all the nitrogen in the wheat straw as protein nitrogen 
indicates a protein content of 3.14 per cent. In a similar manner, calcu¬ 
lating the nitrogen in the straw solution as protein nitrogen indicates an 
average protein content of the straw from the two cultures of 11.29 
cent, thus giving a net gain of 8.15 per cent protein. 
However, but little of this net gain of protein can be attributed to the 
influence of the straw, for an average net gain of only 2.07 mgm. of 
nitrogen per gram of straw was noted in the straw dextrose media. 
A summary of both experiments indicates an average net gain of only 
1.54 mgm. of nitrogen per gram of straw when added to dextrose medium. 
In another experiment three cultures were aerated for four days in a 
molasses solution and three other cultures in a straw-molasses solution. 
The results are recorded in Table VIII. 
Table VIII ,—Effect of straw and molasses on azofication 
Culture No. 
Molasses medium (200 cc.). 
Molasses and straw medium (200 cc.). 
Nitrogen in 
control. 
Total gross 
nitrogen. 
Net nitro¬ 
gen. 
Nitrogen in 
control. 
Total gross 
nitrogen. 
Net nitro¬ 
gen. 
Mgm. 
Mgm. 
Mgm. 
Mgm. 
Mgm. 
Mgm. 
I B. 
17- 54 
19.69 
2. 15 
25.37 
28. 98 
3. 6 i 
10 B.. 
17 - 54 
21.50 
3 - 96 
25-37 
33-88 
8.51 
232 . 
17 - 54 
18. S 7 
■ 1.03 
25-37 
28. 55 
3-18 
