6i8 
Journal of Agricultural Research 
Vol. XXIV, No. 1 
In addition to the two experiments outlined above, 786 plants were 
grown from rusted seed in one of the rooms in the greenhouse without 
any special attempt to isolate the room to keep outside infection from 
the plants. These were grown to maturity during the winter months 
without the development of rust on any of them. 
]Pio. I.—Diagram of air-washing apparatus for isolated room used for growing rust-infected seed: (a) Hose 
connection; (6) spray nozzle; (c) galvanized iron cylinder; (</) greenhouse gutter into which water 
from spray drained; {e) connection pipe from sprayer to blower; (/) electric blower; {a) floor of green¬ 
house; (A) mouth of blower where air entered the room; (i) air intake; (A) greenhouse wall; (/) green¬ 
house roof. 
A number of writers have suggested the possibility of rust infection 
taking place from urediniospores on the surface of the seed or in the 
soil. To test this possibility, several flats were sown with wheat seed 
that had been covered with viable urediniospores of P. glumarum tritici. 
Chul wheat (C. I. No. 2406) was used for this experiment. About 300 
plants were grown to maturity from seed thus treated. No rust infection 
appeared upon any of them at any time. 
