750 
Journal of Agricultural Research 
Vol. XXIV. No. 
the fact that the seed and treatment were similar to those employed at 
Redfield, S. Dak. Results similarly inconclusive were obtained in 
1922 at Rossyln, Va., from an experiment planned to parallel the one 
at Redfield the same year. It became necessary, therefore, to collect 
in South Dakota, entirely, the data concerning this work. 
The plan of experiment was as follows: Allowance was made for 
duplicate rows of each treatment with each lot of beans and quad¬ 
ruplicate rows of untreated beans; rows were 3 feet apart and approxi¬ 
mately 66 feet long; observations were made on nodule formation and 
the presence of disease. 
Plan of Navy Bean Experiment at Redfield^ S. Dak., in ig22 
REDIflEnD BEANS 
REDEIEED BEANS 
•g 
fn 
Two entirely different lots of beans were employed, consisting of the 
unsorted Redfield seed, the progeny of seed which gave such disastrous 
results in previous experiments, and a lot purchased from a seedsman 
of Washington, D. C., which was sorted with special care to remove 
such beans as gave evidence of harboring the bean wilt organism. 
Yellow spots on &e beans, indicative of the disease, were more prevalent 
in the Redfield seed. 
The two cultures of B. radicicola, bean strain, one of which was 
used in 1920, and both of which were tried in 1921, were supplemented 
by a commercial culture and one from Wisconsin.^ All cultures were 
liquid except the commercial culture, which was agar, but for applica¬ 
tion to seed it was necessary to add water. 
Since it was not known which of two media was used for growing the 
cultures employed in the 1920 and 1921 experiments, it was necessary 
to include both Ashby® broth and soil broth made from a modified 
formula of Lohnis® in the experiment as sterile control media. The 
latter medium, however, was used for the propagation of the cultures 
of the bean nodule organism. Soil from Redfield which had produced 
bean plants with nodules on their roots under greenhouse conditions 
comprised the source of soil inoculation. 
* Kindly supplied by Dr. E. B. Fred, University of Wisconsin, Madison, Wis. 
* Ashby, S. F. somb observations on the assimii^a>tion or atmospheric nitrogen by a prbb 
UVING son, ORGANISM.—AZOTOBACTER CHROOCOCCXJM OF BEIJERINCK. In JOUr. Agr. Sci., V. a, p. 38. 
1907. 
* E6hnis, F. laboratory methods IN agricultural BACTERIOLOGY. Tr. by Wm. Stevenson and 
J. H. Smith, xi, 136 p., 40 fig., 3 pi. 1913. 
