752 
Journal of Agricultural Research 
Vol. XXIV, No. ^ 
Observations on the roots revealed that nodules were quite generally 
absent and that their occasional occurrence seemed to be more closely 
connected to the presence of moisture in the soil than to the application 
of inoculating material. 
CONCLUSION 
It is indicated that a slight application of moisture either in the form 
of water, certain culture media in the broth form or legume bacteria 
culture will cause a stimulation of the bean wilt disease under condi¬ 
tions such as obtain at Redfield, S. Dak. 
If the application of legume bacteria culture to beans is necessary, it is 
indicated that satisfactory results may be obtained by adding this mate¬ 
rial in the form of naturally or artificially inoculated soil in a dry condition 
at the rate of not less than 300 pounds per acre. If only a small amount 
of soil is available, it may be mixed with the seed and sown with them. 
This stimulation of disease organisms is apparently not produced with 
similar treatment under the humid conditions and soils of the Arling¬ 
ton Farm, Rosslyn, Va. 
