950 Journal of Agricultural Research voi. xxiv, No. n 
moving into the kernels at a very rapid rate (75) and during the period 
of decline in moisture content of the grain synthesize into the material 
known as gluten. 
It is contended by Brenchley and Hall {2) that in the filling of the en¬ 
dosperm part of the kernel the material is uniform and always possesses 
the same ratio of nitrogenous to nonnitrogenous material and ash. 
Thatcher (jj, found that the ratio of protein to carbohydrates 
decreased with the progress of the development of the kernel. Regard¬ 
less of this difference in the findings, we should not ignore the possibility 
that changes in composition might be brought about by abnormal 
conditions. 
RELATION or NITROGEN TO PHOSPHORUS 
In the investigations by Brenchley and Hall {2) it has been shown 
that the ash and the phosphoric acid enter the grain simultaneously 
Pig. 2.—Relative time of entrance of phosphoric acid and nitrogen into three varieties of wheat 
with the nitrogen. These results have been confirmed, and the graphs 
for nitrogen and phosphoric acid are shown in figure 2. 
It is evident that not only nitrogen but phosphoric acid as well must 
be available in sufficient quantities in the soil if high-nitrogen wheat is 
to be obtained. It should be further noted that the amount of nitrogen 
entering into the kernels at the earliest stages of development is pro¬ 
portionally larger than is the case in subsequent stages of development. 
The embryo is developed and perfected in this early stage and undoubt¬ 
edly the larger amount of nitrogen required at this period is for the con¬ 
struction of the embryo. Following the development of the embryo we 
observe that the phosphoric acid runs parallel with the nitrogen content 
of the kernel. 
