June x 6 ,1923 
Determination of Nicotine 
969 
Table V .—Percentage of nicotine in tobacco extracts and products 
No. 
Brand. 
Guaranty. 
Direct 
precipi¬ 
tation 
method. 
Chapin's 
silico- 
tungstic 
acid 
method.^ 
Improved 
method. 
438892 
16.42 
18.77 
17.31 
317.44 
15.96 
16. 71 
80054 
800 s 5 
80056 
80064 
“Nico-Fume”. 
40.00 
4.00 
30.00 
5.00 
41.60 
4.26 
31.67 
5-13 
17.38 
40.78 
4.27 
30.85 
18 
16.34 
40.77 
4.25 
30.78 
*4.89 
■‘4.89 
'‘Hammon 4 ^s Tobacco Extract”. 
”Nikoteen”. 
" Thompson’s Rose-Nicotine ” . 
* 5‘26 
80065 
80066 
.18 
.72 
4.78 
5.22 
•13 
3-70 
"3.82 
4.89 
. 12 
3-57 
4 ^ 
* 2. <I 
^ 3-51 
80356 
80357 
40358 
80823 
80824 
Hall’s Nicotine Solution”. 
s 0 
S'S 8 8 
40.43 
40.15 
3-76 
^ 40.62 
3 39.82 
^ 13.26 
40.00 
« 2.47 
2.50 
3-51 
39-69 
39.88 
13.18 
40.13 
1.65 
"‘Hall’s Nicotine Sulfate”. 
“Hall’s Nicotine Fumigator”. 
“Black Leaf 40”. 
40.54 
1.64 
80826 
94.63 
94.70 
2.49 
02. jL 2 
Vo / 0 
! 93-75 
^ 94.60 
yo - 
93.60 
94.67 
40.61 
40.73 
94 -14 
80828 
40.00 
94.03 
® 20 . 7/1 
93*72 
AO. 20 
® 40 .i 9 
40.88 
80829 
“Black Leaf 40”. 
40.00 
40.67 
42.21 
39.97 
*41.87 
40.64 
42.09 
^ In this method, the following weights of sample were distilled with 2 gm. NaOH unless otherwise indi¬ 
cated: 
Samples containing 50 per cent or more of nicotine, 0.6 gm. 
Samples containing 30 to 50 per cent of nicotine, i gm. 
Samples containing 3 to 30 per cent of nicotine, 2 gm. 
Samples containing less than 3 per cent of nicotine, 5 gm. 
* This extract had been prepared for several years and was supposed to contain 16.42 per cent of nicotine, 
2.78 per cent of pyridin and 2.37 per cent of ammonium chlorid. The nicotine guaranty is probably only 
approximate. The results obtained for nicotine by all methods include any pyridin that may have been 
present in the washed precipitate. 
* NaOH used in distillation, 0.60 gm, 
* Duplicates from same ether extraction. 
^ NaOH used in distillation, 0.15 gm. 
® NaOH used in distillation, 0.24 gm. 
^ NaOH used in distillation, 0.90 gm. 
® NaOH used in distillation, 0.30 gm. 
