PIRATE 2 
Tomato stems from inoculation experiments with reference to the migration of the 
crowngall organism in the tissues 
The stem A to C, inclusive, shows that the bacteria traveled through regions water- 
soaked by injury and produced galls some distance from their point of entry. In 
all cases the stems were inoculated only at a. 
A, B.—The stems were bruised from b to c and inoculated at a. Proliferations 
developed over the entire length of the injured area. X2/3 (approximately). 
C. —The stem was frozen from b to c with carbon dioxid and inoculated at a. Galls 
appeared at intervals. X2/3 (approximately.) 
The figures D to G, inclusive, show that the bacteria may travel through some 
portion of the vascular system. 
D. —A section was cut out of the stem and the base of the upper part was submerged 
in a suspension of the crowngall bacteria. The organisms were recovered by cultural 
methods at intervals up the stem, which suggests that they passed up through the 
tracheae. X1/5 (approximately). 
E. —As D except that the stem was frozen and dried with the aim of preventing the 
passage of the bacteria up the stem except through the dead vessels. The bacteria 
were recovered in culture from the stem above the frozen region, indicating that they 
had passed through some part of the vascular tissue. X1/5 (approximately). 
F. —A cup, in which a suspension of bacteria was placed, was made from cork, rubber 
tubing, and vaseline. An incision was made in the stem under the surface of the 
suspension and sterile cuts made above and below. X1/5 (approximately). 
G. —A stem inoculated as in F. The cup surrounded the stem at d. The sterile 
cuts which produced galls appear at b. It seems likely that the bacteria which 
produced the galls at b passed through some part of the vascular tissue. X2/5 (ap¬ 
proximately). 
H. I.—Stems severely wounded and inoculated by punctures which ruptured 
some of the vascular bundles at d and a. Sterile punctures were made at intervals 
above. Galls developed at b which were produced by bacteria which probably 
passed through' some of the vascular elements, and were permitted to escape by the 
punctures. H, X2/5 (approximately); I, X1/2. 
48109—23 - 2 
