uly 21, 1923 
Bacterial Spot of Lima Bean 
147 
FERMI’s SOLUTION 
A thin fimbriate pellicle was formed and sank to form a flocculent 
precipitate. Heavy uniform clouding occurred within 3 to 4 days. 
The medium became milky fluorescent within the first 3 or 4 days and 
later became a bluish green color by reflected light. 
uschinsky’s solution 
The action was similar to that in Fermi’s solution except the pellicle 
was more viscid and the bluish color was less prominent. 
litmus agar with sugars 
Dextrose, maltose, and lactose, respectively, were sterilized in concen¬ 
trated water solution and added to sterilized litmus agar in sufficient 
quantities to make a 1 per cent sugar solution. Five cc. of a saturated 
water solution of azolitmin was added to each liter of the neutral nutrient 
agar. 
Litmus lactose and litmus maltose agar slopes produced good growth. 
A very faint reduction of litmus occurred at the bottom of the slope after 
2 weeks, but the normal color soon reappeared and continued exactly 
like the controls throughout the duration of the experiment, about 3 
months. 
On litmus-dextrose agar slopes there was abundant growth and dis¬ 
tinct acid reaction. In 3 days the medium began to redden underneath 
the growth at the base of the slope and this coloration progressed for 
some depth into the medium. Reduction began underneath the heaviest 
growth and was complete within 2 weeks. The medium was then an 
amber or reddish brown color. After about 17 days the litmus color com¬ 
pletely reappeared and remained about the same color as the controls. 
dunham’s solution 
Moderate uniform clouding developed in 3 days. No ring or pellicle 
developed. A flaky precipitate formed which broke up when agitated. 
relation to oxygen 
The organism is aerobic. No growth developed in agar in Roux tubes. 
Tubes of nutrient agar were also inoculated while melted, and rolled to 
distribute the organism throughout. No growth developed at a depth 
greater than 1 mm. below the surface. No clouding developed in the 
closed arm of fermentation tubes with any of the sugars tested. 
fermentation tubes 
The tests were made in 2 per cent peptone solution (+12 Fuller’s 
scale, P H . 8.2 to 8.4) with 2 per cent, respectively, of each of the following 
carbon compounds: glycerine, mannit, dextrose, lactose, maltose, and 
saccharose. 
The sugars and broth were sterilized separately at 12 pounds pressure 
and the desired quantities mixed in the culture tubes and flasks under 
aseptic conditions. Eight tubes were used for each sugar. Clouding 
began after 24 hours. At the end of 6 days heavy clouding was noted 
in the open end of all tubes and a precipitate later developed. In all cases 
there was a definite line of demarcation across the inner part of the U 
