68 
Journal of Agricultural Research 
Vol. XXIX, No. 2 
of the Society of American Bacteriolo¬ 
gists its group number is 5322-31135- 
1333. 
SUMMARY 
(1) The present investigation con¬ 
cerns a leafspot disease of soybean 
called bacterial pustule, which is gener¬ 
ally prevalent in North Carolina. It 
is known to occur also in Texas, Loui¬ 
siana, South Carolina, Virginia, and 
Kansas. 
(2) The disease is distinct from bac¬ 
terial blight and from the diseases of 
bacterial origin which have been des¬ 
cribed in the Orient. 
(3) Bacterial pustule appears to be 
confined to the foliage. Lesions are 
manifested by the presence of pustular 
outgrowths on either or both leaf sur¬ 
faces. They are light green at first, but 
at maturity collapse and become dry and 
reddish brown, and the tissues surround¬ 
ing the lesions become chlorotic. 
(4) The disease is caused by an 
organism to which the name Bac¬ 
terium phaseoli var. sojense was first 
tentatively given by Miss Hedges. 
This organism is herein fully described 
and found to be morphologically and 
culturally indistinguishable from Bad. 
phaseoli E. F. Smith. It forms yellow¬ 
ish colonies on nutrient agar, is flagel¬ 
late, is unable to utilize any of the 
carbon compounds tested except starch 
but can utilize a number of proteins 
including gelatin, casein, blood serum, 
and asparagin. According to the 
descriptive chart of 1920 of the 
American Society of Bacteriologists, 
its group number is 5322-31135-1333. 
(5) When the organism in watery 
suspension is applied to uninjured 
soybean, foliage infection is evident 
within four to six days. Under the 
same conditions of inoculation garden 
beans and Lima beans failed to become 
infected. 
(6) The parasite gains entrance 
through the stomates and passes 
thence into the intercellular spaces. 
The pustules arise by hypertrophic 
changes of any of the parenchymatous 
tissues. 
LITERATURE CITED 
(1) COERPER, F. M. 
1919. BACTERIAL BLIGHT OF SOYBEAN. Jour. 
Agr. Research 18: 179-194, illus. 
(2) Cromwell, R. O. 
1917. FUSARIUM-BLIGHT, OR WILT DISEASE, 
of the soybean. Jour. Agr. Research 8: 
421-439, illus. 
(3) - 
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(4) Hedges, F. 
1922. BACTERIAL PUSTULE OF SOYBEAN. 
Science 56: 111-112. 
(5) - 
1924. SOYBEAN PUSTULE. COMPARATIVE 
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SOJENSE HEDGES AND BACTERIUM PHASEOLI 
e. f. s. (Abstract) Phytopathology 14: 
27-28. 
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